Detailed information NTG0197269

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: A4W88_RS06865
• Genome accession: NZ_CP017275
• Coordinates: 1328865..1329377 (-)
• Length: 170 a.a.
• NCBI ID: WP_041820873.1
• Uniprot ID: A0AAX0VCR7
• Organism: Latilactobacillus sakei strain TMW 1.1398
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1323865..1334377

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W88_RS06835 (A4W88_06775)	-	1324791..1325156 (+)	366	WP_076632064.1	DUF805 domain-containing protein	-
A4W88_RS06845 (A4W88_06785)	-	1325699..1326157 (-)	459	WP_011374992.1	hypothetical protein	-
A4W88_RS06850 (A4W88_06790)	-	1326212..1327408 (-)	1197	WP_011374993.1	acetate kinase	-
A4W88_RS06855 (A4W88_06795)	-	1327430..1328440 (-)	1011	WP_011374994.1	class I SAM-dependent methyltransferase	-
A4W88_RS06860 (A4W88_06800)	-	1328564..1328902 (-)	339	WP_011374995.1	hypothetical protein	-
A4W88_RS06865 (A4W88_06805)	comGF	1328865..1329377 (-)	513	WP_041820873.1	competence type IV pilus minor pilin ComGF	Machinery gene
A4W88_RS06870 (A4W88_06810)	comGE	1329352..1329657 (-)	306	WP_025016385.1	hypothetical protein	Machinery gene
A4W88_RS06875 (A4W88_06815)	comGD	1329644..1330096 (-)	453	WP_076632063.1	competence type IV pilus minor pilin ComGD	Machinery gene
A4W88_RS06880 (A4W88_06820)	comGC	1330068..1330367 (-)	300	WP_076632062.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
A4W88_RS06885 (A4W88_06825)	comGB	1330364..1330876 (-)	513	WP_251930849.1	type II secretion system F family protein	Machinery gene
A4W88_RS06890 (A4W88_06830)	comGB	1331002..1331370 (-)	369	WP_251930850.1	type II secretion system F family protein	Machinery gene
A4W88_RS06895 (A4W88_06835)	comGA	1331363..1332253 (-)	891	WP_076632060.1	competence type IV pilus ATPase ComGA	Machinery gene
A4W88_RS06900 (A4W88_06840)	-	1332369..1333100 (-)	732	WP_076632059.1	YebC/PmpR family DNA-binding transcriptional regulator	-
A4W88_RS06905 (A4W88_06845)	-	1333191..1333691 (-)	501	WP_076632058.1	VanZ family protein	-
A4W88_RS06910 (A4W88_06850)	-	1333788..1334177 (+)	390	WP_076632056.1	hypothetical protein	-

Sequence

Protein

• Length: 170 a.a.
• Molecular weight: 19734.66 Da
• Isoelectric Point: 6.9902

>NTDB_id=197269 A4W88_RS06865 WP_041820873.1 1328865..1329377(-) (comGF) [Latilactobacillus sakei strain TMW 1.1398]
MRLIGRPIKHSAFTLIEMVISMAILILTSQLLVATVGVLQKAIKVTQQQQNTINWHISTIQLDQFLADAYFTHHQDQDGS
KITFRKPDTNQSGEEAETVYYLKRMKNEMVVVSQLDGYMPLLGKLKQLQLDYQRPFVQIDTTFTDQSRYQHEVFLEEQDE
QAKKKTIKTD

Nucleotide

• Length: 513 bp

>NTDB_id=197269 A4W88_RS06865 WP_041820873.1 1328865..1329377(-) (comGF) [Latilactobacillus sakei strain TMW 1.1398]
TTGCGATTAATTGGACGACCGATTAAGCATTCAGCGTTCACGCTAATTGAAATGGTGATTTCAATGGCGATTCTAATCCT
GACGAGTCAGTTACTGGTGGCAACGGTGGGTGTTTTACAAAAGGCAATTAAAGTGACACAACAACAGCAAAATACAATCA
ATTGGCATATCAGTACGATTCAGTTAGATCAGTTTTTAGCCGATGCGTATTTTACACATCATCAGGATCAAGATGGCTCC
AAAATCACTTTTCGAAAACCAGACACTAATCAGTCAGGGGAGGAAGCAGAGACTGTTTACTATTTAAAAAGGATGAAAAA
TGAAATGGTTGTTGTCAGTCAATTAGATGGGTATATGCCATTATTAGGCAAACTCAAGCAACTTCAACTTGACTATCAGC
GACCGTTTGTTCAAATCGATACGACTTTTACGGATCAGAGTCGCTACCAACACGAAGTTTTCTTGGAGGAACAAGATGAA
CAGGCTAAAAAAAAGACGATCAAAACCGATTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (40-149)

• Prokaryotic N-terminal methylation motif (N_methyl) (9-25)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Latilactobacillus sakei subsp. sakei 23K	100	100	1