Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4W87_RS04370 Genome accession   NZ_CP017273
Coordinates   850443..851150 (+) Length   235 a.a.
NCBI ID   WP_231952379.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.1396     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845443..856150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W87_RS04340 (A4W87_04295) - 845654..846841 (+) 1188 WP_011374777.1 FtsW/RodA/SpoVE family cell cycle protein -
  A4W87_RS04345 (A4W87_04300) - 846852..847940 (+) 1089 WP_076632421.1 CAP-associated domain-containing protein -
  A4W87_RS04350 (A4W87_04305) - 847964..848254 (+) 291 WP_076632420.1 YlbG family protein -
  A4W87_RS04355 (A4W87_04310) rsmD 848259..848813 (+) 555 WP_011374774.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A4W87_RS04360 (A4W87_04315) coaD 848826..849323 (+) 498 WP_011374773.1 pantetheine-phosphate adenylyltransferase -
  A4W87_RS04365 (A4W87_04320) - 849325..850371 (+) 1047 WP_011374772.1 SepM family pheromone-processing serine protease -
  A4W87_RS04370 (A4W87_04325) comEA 850443..851150 (+) 708 WP_231952379.1 helix-hairpin-helix domain-containing protein Machinery gene
  A4W87_RS04375 (A4W87_04330) - 851208..851696 (+) 489 WP_011374770.1 ComE operon protein 2 -
  A4W87_RS04380 (A4W87_04335) comEC 851893..853959 (+) 2067 WP_251932818.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A4W87_RS04385 (A4W87_04340) holA 853956..854984 (+) 1029 WP_076632418.1 DNA polymerase III subunit delta -
  A4W87_RS04390 (A4W87_04345) rpsT 855053..855307 (-) 255 WP_011374767.1 30S ribosomal protein S20 -
  A4W87_RS04395 (A4W87_04350) rpsO 855586..855855 (+) 270 WP_011374766.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 235 a.a.        Molecular weight: 26146.73 Da        Isoelectric Point: 9.7682

>NTDB_id=197223 A4W87_RS04370 WP_231952379.1 850443..851150(+) (comEA) [Latilactobacillus sakei strain TMW 1.1396]
MMWQDYWQRYRRFWWVPCLVLLGIIAGGFYWHQSQQKQKVTQNEQLLATSASKTKPATKKTHQAATKKKGGFVDIKGAVV
YPGIYPITVSETRLFEVLKNAGGPTDAADTKRLNLAQKLTDQLIIYIPKKGEVVTDQELIQAPQQIATTSERTQEQASTG
TSANASSTSDTAKIDLNQADSTQLQTLNGIGPKKAEQIIAYRDEQGKFKQIEDLQKVGGIGPKTFEQLQSQICVN

Nucleotide


Download         Length: 708 bp        

>NTDB_id=197223 A4W87_RS04370 WP_231952379.1 850443..851150(+) (comEA) [Latilactobacillus sakei strain TMW 1.1396]
ATGATGTGGCAAGATTATTGGCAGCGATATCGGCGTTTTTGGTGGGTGCCTTGCTTAGTGCTATTGGGGATCATCGCGGG
TGGCTTTTATTGGCATCAATCGCAACAAAAACAAAAAGTAACGCAAAATGAGCAACTGCTAGCAACCAGTGCGTCTAAAA
CAAAACCCGCTACTAAGAAGACACACCAAGCGGCAACCAAGAAAAAAGGCGGTTTTGTCGATATAAAAGGCGCGGTGGTC
TACCCGGGAATTTATCCGATCACAGTCTCTGAAACGCGCTTATTTGAGGTGCTCAAAAATGCAGGTGGTCCGACTGATGC
GGCTGATACCAAGCGGCTTAATTTAGCTCAAAAGTTAACCGATCAACTGATTATTTATATTCCGAAAAAAGGAGAGGTGG
TTACTGATCAAGAACTCATTCAAGCGCCACAACAAATCGCAACGACCAGTGAGCGTACGCAAGAACAGGCATCAACTGGG
ACAAGTGCGAATGCATCGAGCACGAGTGACACTGCTAAAATCGATTTGAATCAGGCTGATAGTACGCAGCTTCAGACCCT
CAATGGCATTGGCCCCAAGAAAGCTGAACAAATAATTGCTTATCGTGATGAACAAGGAAAATTTAAACAGATTGAAGATC
TACAAAAAGTCGGCGGGATTGGTCCGAAGACGTTTGAACAATTGCAATCACAAATTTGTGTGAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(73-124)

Helix-hairpin-helix motif (HHH_3)

(172-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

100

100

1
  comEA Lactococcus lactis subsp. cremoris KW2

36.797

98.298

0.362

Multiple sequence alignment