Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BHY08_RS09425 Genome accession   NZ_CP017267
Coordinates   1960479..1961849 (-) Length   456 a.a.
NCBI ID   WP_071457617.1    Uniprot ID   A0A1J0A7X7
Organism   Vagococcus teuberi strain DSM 21459     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1955479..1966849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHY08_RS09405 (BHY08_09405) - 1955714..1956133 (-) 420 WP_071457613.1 Mini-ribonuclease 3 -
  BHY08_RS09410 (BHY08_09410) cysS 1956130..1957548 (-) 1419 WP_071457614.1 cysteine--tRNA ligase -
  BHY08_RS09415 (BHY08_09415) gltX 1957797..1959272 (-) 1476 WP_071457615.1 glutamate--tRNA ligase -
  BHY08_RS09420 (BHY08_09420) - 1959374..1960459 (-) 1086 WP_071457616.1 PIN/TRAM domain-containing protein -
  BHY08_RS09425 (BHY08_09425) radA 1960479..1961849 (-) 1371 WP_071457617.1 DNA repair protein RadA Machinery gene
  BHY08_RS09430 (BHY08_09430) - 1961959..1962486 (-) 528 WP_071457618.1 dUTP diphosphatase -
  BHY08_RS09435 (BHY08_09435) - 1962586..1964556 (-) 1971 WP_071457888.1 KUP/HAK/KT family potassium transporter -
  BHY08_RS09440 (BHY08_09440) proB 1964804..1965610 (+) 807 WP_071457619.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49957.36 Da        Isoelectric Point: 6.7506

>NTDB_id=197112 BHY08_RS09425 WP_071457617.1 1960479..1961849(-) (radA) [Vagococcus teuberi strain DSM 21459]
MAKKKRSEYVCQTCGYISPKYLGRCPNCGEWNTLVEEIASEVPTRHSRVSLTGEVSKPQKLQEVTAQKEPRVKTQLGELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLSLLKGTVLYVSGEESSQQIKMRAERLADGSNDFYVYPETDMGRIKQ
VIEQLTPDYVIIDSIQTMVHPETESAAGSVSQVRANTAELMQIAKTNNIAIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMRQAGLVEVLNPSEAFLEERIDGATGSAIVCSMEGTRPILAEIQSLVTPSVFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAVGGVKLDEPAIDLSIAVSIASSYKDKGTSPQDCFIGEIGLTG
EIRRVNRIEQRVGEAQKLGFKRVFIPINNLQGWTPPKDIEVIGVETLSETLFKVFS

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=197112 BHY08_RS09425 WP_071457617.1 1960479..1961849(-) (radA) [Vagococcus teuberi strain DSM 21459]
TTGGCAAAGAAAAAAAGAAGTGAATATGTCTGTCAAACATGTGGCTATATTTCCCCAAAGTATTTAGGTAGGTGTCCTAA
TTGCGGAGAGTGGAATACTTTAGTTGAAGAAATCGCCTCAGAGGTTCCAACTAGACATAGCAGAGTTAGTTTGACAGGTG
AAGTGAGCAAACCCCAAAAACTTCAAGAAGTGACTGCTCAAAAAGAGCCACGGGTTAAAACACAGTTAGGTGAGTTAAAT
CGTGTGTTAGGTGGAGGAGTTGTCCCAGGGTCTATGGTGTTAATTGGTGGAGACCCTGGTATTGGAAAATCTACTTTACT
ATTACAAGTGTCTCAACAACTAAGCTTATTAAAGGGAACTGTGTTATATGTTTCTGGTGAAGAAAGTTCACAACAAATAA
AAATGCGTGCGGAACGCTTGGCTGATGGTAGCAATGATTTTTATGTTTATCCTGAAACAGATATGGGACGAATCAAGCAA
GTTATTGAGCAATTAACACCAGATTATGTGATTATTGATTCGATTCAAACGATGGTTCATCCTGAAACAGAGAGTGCTGC
TGGAAGTGTGAGTCAAGTTCGAGCGAATACGGCTGAGTTAATGCAAATTGCTAAAACAAACAATATTGCCATCTTCATTG
TAGGACACGTGACGAAAGAAGGCTCGCTTGCAGGACCACGGATGCTTGAACATATGGTGGATACAGTACTTTATTTTGAA
GGAGATAGACACCATACTTTCAGAATACTGCGTGCTGTAAAAAATCGTTTTGGCTCAACAAATGAGATTGGTATTTTTGA
AATGAGGCAAGCTGGATTAGTAGAAGTGTTAAATCCATCAGAAGCATTTTTAGAAGAGCGAATTGACGGGGCGACAGGTT
CAGCGATTGTATGTTCGATGGAAGGTACGCGTCCTATTTTGGCAGAGATACAATCACTTGTAACGCCATCTGTTTTTGGA
AATGCTAAACGAACGGCAACAGGGCTAGATTACAACCGTGTATCACTGATTATGGCTGTGTTAGAAAAACGTGCTGGATT
ATTATTACAAAACCAAGATGCTTATTTAAAAGCAGTTGGTGGAGTGAAGCTTGATGAACCAGCGATTGATTTATCCATCG
CAGTGAGTATCGCATCAAGTTACAAAGATAAAGGGACTTCTCCTCAAGATTGTTTTATTGGTGAAATTGGGTTAACTGGT
GAAATTAGACGAGTGAACCGAATTGAACAGCGTGTTGGCGAAGCACAAAAATTAGGCTTCAAACGTGTGTTTATTCCAAT
AAATAATCTGCAAGGTTGGACACCACCTAAAGACATTGAAGTGATTGGTGTCGAAACATTATCAGAAACATTATTTAAAG
TATTTTCTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-221)

Subunit ChlI of Mg-chelatase (ChlI)

(366-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J0A7X7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

73.684

100

0.737
  radA Streptococcus mitis SK321

73.465

100

0.735
  radA Streptococcus pneumoniae Rx1

73.465

100

0.735
  radA Streptococcus pneumoniae D39

73.465

100

0.735
  radA Streptococcus pneumoniae R6

73.465

100

0.735
  radA Streptococcus pneumoniae TIGR4

73.465

100

0.735
  radA Bacillus subtilis subsp. subtilis str. 168

67.108

99.342

0.667

Multiple sequence alignment