Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   CDH89_RS00390 Genome accession   NZ_CP021844
Coordinates   89622..90290 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain EC1515     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 84622..95290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDH89_RS00355 (CDH89_00355) yicC 84641..85504 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  CDH89_RS00365 (CDH89_00365) rph 85631..86347 (+) 717 WP_001247089.1 ribonuclease PH -
  CDH89_RS00370 (CDH89_00370) pyrE 86413..87054 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  CDH89_RS00375 (CDH89_00375) slmA 87091..87687 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  CDH89_RS00380 (CDH89_00380) dut 87794..88252 (-) 459 WP_000976070.1 dUTP diphosphatase -
  CDH89_RS00385 (CDH89_00385) coaBC 88230..89450 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  CDH89_RS00390 (CDH89_00390) radC 89622..90290 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  CDH89_RS00395 (CDH89_00395) rpmB 90507..90743 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  CDH89_RS00400 (CDH89_00400) rpmG 90764..90931 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  CDH89_RS00405 (CDH89_00405) mutM 91029..91838 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  CDH89_RS00410 (CDH89_00410) coaD 91877..92356 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  CDH89_RS00415 (CDH89_00415) waaA 92364..93641 (-) 1278 WP_000891562.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  CDH89_RS00420 (CDH89_00420) rfaQ 94054..95112 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=196646 CDH89_RS00390 WP_001297375.1 89622..90290(+) (radC) [Escherichia coli strain EC1515]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=196646 CDH89_RS00390 WP_001297375.1 89622..90290(+) (radC) [Escherichia coli strain EC1515]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAATTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496