Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BHS01_RS02180 Genome accession   NZ_CP017195
Coordinates   420329..421693 (+) Length   454 a.a.
NCBI ID   WP_047916078.1    Uniprot ID   A0A7L4WEA7
Organism   Lactococcus paracarnosus strain TMW 2.1615     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 415329..426693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BHS01_RS02160 (BHS01_02145) - 415727..417445 (+) 1719 WP_109835072.1 ABC transporter ATP-binding protein -
  BHS01_RS02165 (BHS01_02150) - 417438..419189 (+) 1752 WP_109835071.1 ABC transporter ATP-binding protein -
  BHS01_RS02170 (BHS01_02155) - 419443..419889 (+) 447 WP_109835070.1 dUTP diphosphatase -
  BHS01_RS02175 (BHS01_02160) - 419840..420319 (+) 480 WP_162542441.1 GNAT family N-acetyltransferase -
  BHS01_RS02180 (BHS01_02165) radA 420329..421693 (+) 1365 WP_047916078.1 DNA repair protein RadA Machinery gene
  BHS01_RS02185 (BHS01_02170) - 422009..423082 (+) 1074 WP_109835068.1 PIN/TRAM domain-containing protein -
  BHS01_RS02190 (BHS01_02175) ispD 423079..423756 (+) 678 WP_109835067.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BHS01_RS02195 (BHS01_02180) ispF 423791..424276 (+) 486 WP_109835066.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BHS01_RS02200 (BHS01_02185) gltX 424403..425851 (+) 1449 WP_109835065.1 glutamate--tRNA ligase -
  BHS01_RS02205 (BHS01_02190) - 425887..426471 (-) 585 WP_233220362.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49347.04 Da        Isoelectric Point: 6.7240

>NTDB_id=196562 BHS01_RS02180 WP_047916078.1 420329..421693(+) (radA) [Lactococcus paracarnosus strain TMW 2.1615]
MAKIKSKFVCQSCGYQSAKYLGRCPNCGAWSSFVEEVEAKAVKNARVTLTGEKTKPIKLGEVESLDTPRVLTEMGEFNRV
LGGGVVPGSLILIGGDPGIGKSTILLQVSVQLANLGRVLYVSGEESAQQIKMRAERLGDLDSDFYLYAENNMQNIRAEIE
KLQPDFLVIDSIQTVMSPEITGVQGSVSQVREVTAELLQIAKTNNIATFIVGHVTKEGSLAGPRMLEHMVDTVLYFEGER
QHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVTNPSEIFLEERLEGATGSAIVVSMEGTRPILVEIQCLVTPTVFGNAR
RTTSGLDFNRVSLIMAVLEKRANLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIVSSYKEKPTQPDVAFIGEIGLTGEIR
RVNRIESRINEAAKLGFKKIYVPKNALVGLAIPSSIKVVGVTTLAEVLQKVFGA

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=196562 BHS01_RS02180 WP_047916078.1 420329..421693(+) (radA) [Lactococcus paracarnosus strain TMW 2.1615]
ATGGCTAAAATAAAATCAAAATTCGTCTGTCAGTCTTGTGGCTACCAGTCTGCTAAGTATTTAGGAAGATGTCCAAACTG
TGGTGCTTGGAGTTCATTTGTTGAAGAGGTAGAAGCTAAGGCAGTCAAAAATGCTCGGGTTACCTTAACTGGCGAAAAAA
CAAAACCCATTAAATTAGGGGAGGTCGAAAGTTTAGATACGCCACGTGTGCTAACGGAGATGGGAGAGTTTAATCGCGTT
CTAGGTGGGGGCGTGGTACCTGGTAGTTTAATTCTCATTGGTGGCGATCCAGGGATCGGAAAATCAACGATTCTCTTACA
GGTTTCAGTTCAACTTGCAAATCTTGGCCGTGTCTTATATGTATCTGGAGAAGAATCTGCCCAACAAATTAAGATGCGGG
CTGAACGCCTAGGTGATTTAGACTCAGACTTCTATCTATACGCTGAAAACAACATGCAAAACATTCGGGCGGAAATCGAG
AAATTACAACCTGATTTTCTCGTCATAGACTCGATTCAAACAGTCATGAGCCCAGAAATTACAGGGGTTCAAGGCTCTGT
TAGTCAGGTTCGAGAAGTCACTGCTGAACTGCTTCAGATTGCAAAAACGAATAATATCGCGACCTTCATCGTCGGACATG
TTACCAAAGAGGGTAGCCTTGCGGGACCTAGAATGCTAGAACATATGGTAGATACGGTGCTCTATTTTGAAGGCGAACGA
CAACATACTTTTCGTATTTTGAGAGCTGTGAAAAATCGCTTTGGCTCGACGAATGAGATTGGTATTTTTGAGATGCAGTC
ACAGGGGCTTGTTGAAGTGACAAATCCTAGTGAGATTTTCTTAGAAGAGCGTTTGGAAGGTGCAACAGGGTCTGCCATCG
TCGTATCGATGGAGGGGACACGTCCTATCCTTGTTGAAATCCAGTGTCTGGTCACGCCGACAGTTTTTGGAAATGCGAGA
CGAACGACATCAGGTCTCGATTTTAATCGGGTTAGTTTGATTATGGCTGTCCTTGAAAAACGTGCAAATTTACTACTTCA
AAATCAGGATGCCTATCTCAAGTCAGCTGGTGGTGTTAAATTAGATGAGCCAGCTATCGATCTGGCAGTTGCGGTCGCGA
TTGTCTCAAGTTATAAGGAGAAACCAACACAACCAGATGTTGCTTTCATCGGTGAAATTGGTTTGACAGGTGAAATCAGG
CGTGTGAATCGGATTGAAAGTCGGATTAATGAAGCTGCTAAACTAGGCTTTAAAAAAATCTATGTACCCAAAAATGCATT
AGTAGGCTTGGCTATACCATCATCAATTAAAGTTGTTGGTGTCACAACATTAGCTGAAGTTTTACAAAAAGTTTTTGGTG
CTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(78-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7L4WEA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.858

99.559

0.815
  radA Streptococcus pneumoniae D39

81.858

99.559

0.815
  radA Streptococcus pneumoniae R6

81.858

99.559

0.815
  radA Streptococcus pneumoniae TIGR4

81.858

99.559

0.815
  radA Streptococcus mitis SK321

81.858

99.559

0.815
  radA Streptococcus mitis NCTC 12261

81.637

99.559

0.813
  radA Bacillus subtilis subsp. subtilis str. 168

63.239

100

0.637

Multiple sequence alignment