Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BFV65_RS18805 Genome accession   NZ_CP017186
Coordinates   3989479..3990912 (+) Length   477 a.a.
NCBI ID   WP_022649530.1    Uniprot ID   A0AAI9D3Q5
Organism   Enterobacter hormaechei subsp. hoffmannii strain DSM 14563     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3984479..3995912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFV65_RS18775 (BFV65_18775) - 3985046..3986023 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  BFV65_RS18780 (BFV65_18780) kdsD 3986037..3987023 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  BFV65_RS18785 (BFV65_18785) kdsC 3987038..3987604 (+) 567 WP_017382729.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  BFV65_RS18790 (BFV65_18790) lptC 3987601..3988176 (+) 576 WP_022649529.1 LPS export ABC transporter periplasmic protein LptC -
  BFV65_RS18795 (BFV65_18795) lptA 3988145..3988699 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  BFV65_RS18800 (BFV65_18800) lptB 3988706..3989431 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  BFV65_RS18805 (BFV65_18805) rpoN/rpoN1 3989479..3990912 (+) 1434 WP_022649530.1 RNA polymerase factor sigma-54 Machinery gene
  BFV65_RS18810 (BFV65_18810) hpf 3990935..3991222 (+) 288 WP_014171818.1 ribosome hibernation promoting factor -
  BFV65_RS18815 (BFV65_18815) ptsN 3991305..3991796 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BFV65_RS18820 (BFV65_18820) rapZ 3991842..3992696 (+) 855 WP_003861870.1 RNase adapter RapZ -
  BFV65_RS18825 (BFV65_18825) npr 3992693..3992965 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  BFV65_RS18830 (BFV65_18830) mtgA 3993005..3993730 (-) 726 WP_069596992.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  BFV65_RS18835 (BFV65_18835) elbB 3993727..3994380 (-) 654 WP_069596993.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54128.02 Da        Isoelectric Point: 4.3409

>NTDB_id=196476 BFV65_RS18805 WP_022649530.1 3989479..3990912(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. hoffmannii strain DSM 14563]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPE
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISEHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=196476 BFV65_RS18805 WP_022649530.1 3989479..3990912(+) (rpoN/rpoN1) [Enterobacter hormaechei subsp. hoffmannii strain DSM 14563]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAACAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCTCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAG
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGATTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ATGAAGCCCGGCTCATCATCAGCGAGCATCTGGATTTGCTGGCAAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTCAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAATCTATCCAGACCAG
CGAACCGGAATACGTTATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAAAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGCCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment