Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LACFE_RS05835 Genome accession   NZ_CP017151
Coordinates   1205196..1206569 (+) Length   457 a.a.
NCBI ID   WP_069776000.1    Uniprot ID   A0A1D7ZXM7
Organism   Limosilactobacillus fermentum strain NCC2970     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1200196..1211569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LACFE_RS05805 (LACFE_CDS1131) - 1200200..1201075 (+) 876 WP_004562728.1 alpha/beta hydrolase -
  LACFE_RS05810 (LACFE_CDS1132) - 1201392..1202729 (-) 1338 WP_004562729.1 aminopeptidase C -
  LACFE_RS05815 (LACFE_CDS1133) - 1202793..1203407 (-) 615 WP_004563340.1 sugar O-acetyltransferase -
  LACFE_RS05820 (LACFE_CDS1134) rpiA 1203435..1204121 (-) 687 WP_014562151.1 ribose-5-phosphate isomerase RpiA -
  LACFE_RS05825 (LACFE_CDS1135) - 1204133..1204444 (-) 312 WP_004562732.1 GNAT family N-acetyltransferase -
  LACFE_RS05830 (LACFE_CDS1136) - 1204644..1205183 (+) 540 WP_004563338.1 dUTP diphosphatase -
  LACFE_RS05835 (LACFE_CDS1137) radA 1205196..1206569 (+) 1374 WP_069776000.1 DNA repair protein RadA Machinery gene
  LACFE_RS05840 (LACFE_CDS1138) - 1206602..1207735 (+) 1134 WP_004563337.1 PIN/TRAM domain-containing protein -
  LACFE_RS05845 (LACFE_CDS1139) gltX 1207806..1209302 (+) 1497 WP_069776001.1 glutamate--tRNA ligase -
  LACFE_RS05850 (LACFE_CDS1140) - 1209370..1209939 (+) 570 WP_004562737.1 TIGR01440 family protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49411.82 Da        Isoelectric Point: 8.7358

>NTDB_id=196067 LACFE_RS05835 WP_069776000.1 1205196..1206569(+) (radA) [Limosilactobacillus fermentum strain NCC2970]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKITLG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=196067 LACFE_RS05835 WP_069776000.1 1205196..1206569(+) (radA) [Limosilactobacillus fermentum strain NCC2970]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACGGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTACCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D7ZXM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.212

99.125

0.676
  radA Streptococcus mitis NCTC 12261

67.991

99.125

0.674
  radA Streptococcus pneumoniae Rx1

67.991

99.125

0.674
  radA Streptococcus pneumoniae D39

67.991

99.125

0.674
  radA Streptococcus pneumoniae R6

67.991

99.125

0.674
  radA Streptococcus pneumoniae TIGR4

67.991

99.125

0.674
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637

Multiple sequence alignment