Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BS16045_RS02010 Genome accession   NZ_CP017112
Coordinates   371617..372066 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain BS16045     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366617..377066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS16045_RS01975 (BS16045_00386) yckA 366888..367567 (-) 680 Protein_341 amino acid ABC transporter permease -
  BS16045_RS01980 (BS16045_00387) yckB 367577..368440 (-) 864 WP_080479507.1 transporter substrate-binding domain-containing protein -
  BS16045_RS22260 - 368602..368798 (+) 197 Protein_343 hypothetical protein -
  BS16045_RS01990 (BS16045_00389) - 368835..369155 (+) 321 Protein_344 RDD family protein -
  BS16045_RS01995 (BS16045_00390) yckD 369237..369569 (+) 333 WP_015252849.1 YckD family protein -
  BS16045_RS02000 (BS16045_00391) bglC 369722..371155 (+) 1434 WP_069322363.1 6-phospho-beta-glucosidase -
  BS16045_RS02005 (BS16045_00392) nin/comJ 371192..371590 (-) 399 WP_015715226.1 DNA-entry nuclease inhibitor Regulator
  BS16045_RS02010 (BS16045_00393) nucA/comI 371617..372066 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  BS16045_RS02015 (BS16045_00394) tlpC 372234..373955 (-) 1722 WP_069322364.1 methyl-accepting chemotaxis protein TlpC -
  BS16045_RS02020 (BS16045_00395) hxlB 374066..374623 (-) 558 WP_069322365.1 6-phospho-3-hexuloisomerase -
  BS16045_RS02025 (BS16045_00396) hxlA 374629..375261 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  BS16045_RS02030 (BS16045_00397) hxlR 375489..375851 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=195663 BS16045_RS02010 WP_014475770.1 371617..372066(-) (nucA/comI) [Bacillus subtilis strain BS16045]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=195663 BS16045_RS02010 WP_014475770.1 371617..372066(-) (nucA/comI) [Bacillus subtilis strain BS16045]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGACACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987

Multiple sequence alignment