Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   EnteroDNA1_RS11480 Genome accession   NZ_CP017087
Coordinates   2420147..2421580 (+) Length   477 a.a.
NCBI ID   WP_047060252.1    Uniprot ID   -
Organism   Enterobacter sp. HK169     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2415147..2426580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EnteroDNA1_RS11450 (EnteroDNA1_02276) - 2415714..2416691 (+) 978 WP_023309354.1 calcium/sodium antiporter -
  EnteroDNA1_RS11455 (EnteroDNA1_02277) kdsD 2416705..2417691 (+) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  EnteroDNA1_RS11460 (EnteroDNA1_02278) kdsC 2417706..2418272 (+) 567 WP_048981180.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  EnteroDNA1_RS11465 (EnteroDNA1_02279) lptC 2418269..2418844 (+) 576 WP_069303345.1 LPS export ABC transporter periplasmic protein LptC -
  EnteroDNA1_RS11470 (EnteroDNA1_02280) lptA 2418813..2419367 (+) 555 WP_033146750.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  EnteroDNA1_RS11475 (EnteroDNA1_02281) lptB 2419374..2420099 (+) 726 WP_033146751.1 LPS export ABC transporter ATP-binding protein -
  EnteroDNA1_RS11480 (EnteroDNA1_02282) rpoN/rpoN1 2420147..2421580 (+) 1434 WP_047060252.1 RNA polymerase factor sigma-54 Machinery gene
  EnteroDNA1_RS11485 (EnteroDNA1_02283) hpf 2421603..2421890 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  EnteroDNA1_RS11490 (EnteroDNA1_02284) ptsN 2421973..2422464 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  EnteroDNA1_RS11495 (EnteroDNA1_02285) rapZ 2422510..2423364 (+) 855 WP_014885397.1 RNase adapter RapZ -
  EnteroDNA1_RS11500 (EnteroDNA1_02286) npr 2423361..2423633 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  EnteroDNA1_RS11505 (EnteroDNA1_02287) mtgA 2423673..2424398 (-) 726 WP_069303346.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  EnteroDNA1_RS11510 (EnteroDNA1_02288) elbB 2424395..2425048 (-) 654 WP_069303347.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54197.95 Da        Isoelectric Point: 4.3173

>NTDB_id=195359 EnteroDNA1_RS11480 WP_047060252.1 2420147..2421580(+) (rpoN/rpoN1) [Enterobacter sp. HK169]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=195359 EnteroDNA1_RS11480 WP_047060252.1 2420147..2421580(+) (rpoN/rpoN1) [Enterobacter sp. HK169]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATCCTGGAAAGCATGGGCGACGAAGAGATTGAACTTGAGGAGATCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTGAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAAAGCCGAAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment