Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   FORC47_RS20650 Genome accession   NZ_CP017060
Coordinates   3958838..3959272 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain FORC_047     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3953838..3964272
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC47_RS20635 (FORC47_3933) - 3955015..3955572 (+) 558 WP_016080434.1 PadR family transcriptional regulator -
  FORC47_RS20640 (FORC47_3934) metE 3956085..3958373 (+) 2289 WP_089171703.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  FORC47_RS20645 (FORC47_3935) - 3958412..3958801 (-) 390 WP_000424024.1 competence protein ComJ -
  FORC47_RS20650 (FORC47_3936) nucA/comI 3958838..3959272 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  FORC47_RS20660 (FORC47_3937) - 3959499..3960248 (+) 750 WP_000388467.1 DUF3967 domain-containing protein -
  FORC47_RS20665 (FORC47_3938) kinB 3960287..3961561 (-) 1275 WP_000420372.1 sporulation sensor histidine kinase KinB -
  FORC47_RS20670 (FORC47_3939) - 3961872..3962072 (+) 201 WP_000929276.1 hypothetical protein -
  FORC47_RS20675 (FORC47_3940) - 3962195..3963358 (+) 1164 WP_089171704.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=194877 FORC47_RS20650 WP_000811494.1 3958838..3959272(-) (nucA/comI) [Bacillus cereus strain FORC_047]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=194877 FORC47_RS20650 WP_000811494.1 3958838..3959272(-) (nucA/comI) [Bacillus cereus strain FORC_047]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTGTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment