Detailed information NTG0194857

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FORC47_RS05855
• Genome accession: NZ_CP017060
• Coordinates: 1123320..1127045 (+)
• Length: 1241 a.a.
• NCBI ID: WP_089170590.1
• Uniprot ID: -
• Organism: Bacillus cereus strain FORC_047
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1118320..1132045

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FORC47_RS05840 (FORC47_1018)	-	1118478..1119071 (+)	594	WP_089170588.1	TVP38/TMEM64 family protein	-
FORC47_RS05845 (FORC47_1019)	lepB	1119128..1119691 (+)	564	WP_000751897.1	signal peptidase I	-
FORC47_RS05850 (FORC47_1020)	addB	1119808..1123323 (+)	3516	WP_089170589.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FORC47_RS05855 (FORC47_1021)	addA	1123320..1127045 (+)	3726	WP_089170590.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FORC47_RS05860 (FORC47_1022)	-	1127069..1127347 (+)	279	WP_089170591.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FORC47_RS05865 (FORC47_1023)	gerPF	1127484..1127699 (-)	216	WP_001141570.1	spore germination protein GerPF	-
FORC47_RS05870 (FORC47_1024)	-	1127742..1128128 (-)	387	WP_000902329.1	spore germination protein GerPE	-
FORC47_RS05875 (FORC47_1025)	gerPD	1128144..1128338 (-)	195	WP_001052807.1	spore germination protein GerPD	-
FORC47_RS05880 (FORC47_1026)	gerPC	1128345..1128959 (-)	615	WP_089170592.1	spore germination protein GerPC	-
FORC47_RS05885 (FORC47_1027)	gerPB	1129027..1129233 (-)	207	WP_001012508.1	spore germination protein GerPB	-
FORC47_RS05890 (FORC47_1028)	gerPA	1129248..1129469 (-)	222	WP_001111187.1	spore germination protein GerPA	-
FORC47_RS05895 (FORC47_1029)	-	1129566..1129745 (-)	180	WP_000462857.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FORC47_RS05900 (FORC47_1030)	-	1129994..1130893 (+)	900	WP_053565035.1	fumarylacetoacetate hydrolase family protein	-
FORC47_RS05905 (FORC47_1031)	-	1130928..1131209 (-)	282	WP_000926859.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142652.74 Da
• Isoelectric Point: 4.8146

>NTDB_id=194857 FORC47_RS05855 WP_089170590.1 1123320..1127045(+) (addA) [Bacillus cereus strain FORC_047]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPEGPAPRIETLQADVALLGTLSSAARESWTSLYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVMDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHNEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEG

Nucleotide

• Length: 3726 bp

>NTDB_id=194857 FORC47_RS05855 WP_089170590.1 1123320..1127045(+) (addA) [Bacillus cereus strain FORC_047]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAACCCAGTCGATGTTGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCAGCACAAGAGATGAAAAACCGAATC
GGAGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAACACGTAAGAAAGCAGCTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTTGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGCATCGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAGGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGAAGGTCCAGCGCCTCGCATTGAAACCCTGCAAGCAGATGTAGCTTTACT
TGGAACGCTATCATCAGCTGCTCGTGAGTCGTGGACAAGTTTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAGGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGACAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCTTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTGTTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGCGACGTAAAGCAGTCAATTTATCGTTTCCGACTAGCCG
AACCAGGCTTATTCCTAGGAAAGTATAAACGCTTTACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AATTTTCGTAGTCGTCATGAAGTGCTAGCAGGAACGAACTTCATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCAGAGCTATTATGTA
TTCAGCAAACGGAAGAAGAAGTAATGGATGGAGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTAGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGATTTCGTTATTTTACTCCGCTCGATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCACCGATTGTGGGACTAAATGATGAAGACCTTGCGACGCTTCG
TGCTCATGGGAAGAAAGGGTCATTTTATGAAGTAATGAGCTCGTTCTTAAAAGGAGCACCGCTTGAAGAAGAGCAAGAAC
TTCATGATAAATTAGAGTGGTTTTATAATTTACTGCAAGGATGGCGTGAATTTGCCCGTCAACAATCCCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCAGGAAAGCAAAGACAAGCAAACTT
ACGCGTACTATATGACCGCGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTGCGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCAAGAGCTCTTGGTGAACAAGAAGACGTCGTTCGAATTATGACGATT
CATAAAAGTAAAGGGCTAGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGATTTCGGTTTCGGTTCACAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCAATTAAACGCAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATATGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAATGAATGGTTATTACCAGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCGGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCAATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATCGATATAAAGTGCAACTTTCGTTATATGCAAAAGCACTCGAGAAAAGCTTACAACATCCTGTCAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCACGTTGTAAATATTGAAGGATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.248	100	0.535