Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BGI23_RS20750 Genome accession   NZ_CP017016
Coordinates   3991424..3992170 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus sp. ABP14     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3986424..3997170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BGI23_RS20725 (BGI23_20725) dnaG 3987194..3988990 (-) 1797 WP_000528216.1 DNA primase -
  BGI23_RS20740 (BGI23_20740) - 3989842..3990654 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  BGI23_RS20745 (BGI23_20745) - 3990684..3991316 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  BGI23_RS20750 (BGI23_20750) recO 3991424..3992170 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  BGI23_RS20755 (BGI23_20755) - 3992204..3992347 (-) 144 WP_000883924.1 YqzL family protein -
  BGI23_RS20760 (BGI23_20760) era 3992524..3993429 (-) 906 WP_001080241.1 GTPase Era -
  BGI23_RS20765 (BGI23_20765) - 3993422..3993820 (-) 399 WP_001014347.1 cytidine deaminase -
  BGI23_RS20770 (BGI23_20770) - 3993920..3994273 (-) 354 WP_000715482.1 diacylglycerol kinase family protein -
  BGI23_RS20775 (BGI23_20775) ybeY 3994270..3994740 (-) 471 WP_070806843.1 rRNA maturation RNase YbeY -
  BGI23_RS20780 (BGI23_20780) - 3994737..3996881 (-) 2145 WP_000946450.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=194501 BGI23_RS20750 WP_000487010.1 3991424..3992170(-) (recO) [Bacillus sp. ABP14]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=194501 BGI23_RS20750 WP_000487010.1 3991424..3992170(-) (recO) [Bacillus sp. ABP14]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGCCGGTTAGCATCTGTTTCGCAACTTATGA
CACATGGTCATTTTCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGGGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment