Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BGI23_RS19220 Genome accession   NZ_CP017016
Coordinates   3730989..3731423 (-) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus sp. ABP14     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3725989..3736423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BGI23_RS19205 (BGI23_19205) - 3726991..3727554 (+) 564 WP_000062057.1 PadR family transcriptional regulator -
  BGI23_RS19210 (BGI23_19210) metE 3728234..3730522 (+) 2289 WP_070806772.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BGI23_RS19215 (BGI23_19215) comJ 3730561..3730950 (-) 390 WP_000424015.1 competence protein ComJ -
  BGI23_RS19220 (BGI23_19220) nucA/comI 3730989..3731423 (-) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  BGI23_RS19225 (BGI23_19225) - 3731660..3732409 (+) 750 WP_000388462.1 DUF3967 domain-containing protein -
  BGI23_RS19230 (BGI23_19230) kinB 3732463..3733737 (-) 1275 WP_000420387.1 sporulation sensor histidine kinase KinB -
  BGI23_RS19235 (BGI23_19235) - 3734047..3734247 (+) 201 WP_000929285.1 hypothetical protein -
  BGI23_RS19240 (BGI23_19240) - 3734371..3735528 (+) 1158 WP_000434567.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=194497 BGI23_RS19220 WP_000811499.1 3730989..3731423(-) (nucA/comI) [Bacillus sp. ABP14]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=194497 BGI23_RS19220 WP_000811499.1 3730989..3731423(-) (nucA/comI) [Bacillus sp. ABP14]
ATGAAGCAATTAAAGGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGACATTCAGAAGTGTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCCGATAACCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556

Multiple sequence alignment