Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BGI23_RS00480 Genome accession   NZ_CP017016
Coordinates   93447..94823 (+) Length   458 a.a.
NCBI ID   WP_001085212.1    Uniprot ID   A0A2B3Y2M8
Organism   Bacillus sp. ABP14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 88447..99823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BGI23_RS00460 (BGI23_00460) ctsR 88642..89103 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  BGI23_RS00465 (BGI23_00465) - 89275..89823 (+) 549 WP_044790592.1 UvrB/UvrC motif-containing protein -
  BGI23_RS00470 (BGI23_00470) - 89828..90892 (+) 1065 WP_000050825.1 protein arginine kinase -
  BGI23_RS00475 (BGI23_00475) clpC 90915..93350 (+) 2436 WP_000971177.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BGI23_RS00480 (BGI23_00480) radA 93447..94823 (+) 1377 WP_001085212.1 DNA repair protein RadA Machinery gene
  BGI23_RS00485 (BGI23_00485) disA 94827..95900 (+) 1074 WP_000392171.1 DNA integrity scanning diadenylate cyclase DisA -
  BGI23_RS00490 (BGI23_00490) - 96061..97170 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  BGI23_RS00495 (BGI23_00495) ispD 97187..97867 (+) 681 WP_000288305.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BGI23_RS00500 (BGI23_00500) ispF 97983..98459 (+) 477 WP_000488383.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49858.44 Da        Isoelectric Point: 7.1421

>NTDB_id=194473 BGI23_RS00480 WP_001085212.1 93447..94823(+) (radA) [Bacillus sp. ABP14]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
THIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=194473 BGI23_RS00480 WP_001085212.1 93447..94823(+) (radA) [Bacillus sp. ABP14]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCAAAATATATGGGGAAATGTCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCCAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTAACAGAAGTGGAAACAAAGTCTGAGGCGCGTATTGAAACAAAATTTCAAGAATTT
AACCGTGTACTTGGTGGCGGGATTGTAGATGGATCCTTAGTACTGATTGGTGGAGACCCCGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCAGATTCTTCATATGATGTCCTATATATATCGGGTGAAGAATCAGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACGGATTTACAGCGAATTGCA
ACACATATTGAAGAGGTGAACCCAGCCTTTGTTGTTATTGATTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCGGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGGGCGATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCGG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCATTAATTTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGATGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCGGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCAGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCACTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B3Y2M8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.946

100

0.751
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.576

98.908

0.629

Multiple sequence alignment