Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BC350_RS10305 Genome accession   NZ_CP016914
Coordinates   2139154..2140881 (-) Length   575 a.a.
NCBI ID   WP_011002731.1    Uniprot ID   A0A223GQM6
Organism   Ralstonia pseudosolanacearum strain CQPS-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2134154..2145881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC350_RS10275 (BC350_09990) - 2134778..2135239 (+) 462 WP_011002737.1 NUDIX domain-containing protein -
  BC350_RS10280 (BC350_09995) yacG 2135232..2135447 (-) 216 WP_058908246.1 DNA gyrase inhibitor YacG -
  BC350_RS10285 (BC350_10000) zapD 2135458..2136216 (-) 759 WP_016723794.1 cell division protein ZapD -
  BC350_RS10290 (BC350_10005) coaE 2136334..2136948 (-) 615 WP_011002734.1 dephospho-CoA kinase -
  BC350_RS10295 (BC350_10010) - 2136961..2137842 (-) 882 WP_029240316.1 prepilin peptidase -
  BC350_RS10300 (BC350_10015) pilC 2137845..2139110 (-) 1266 WP_011002732.1 type II secretion system F family protein Machinery gene
  BC350_RS10305 (BC350_10020) pilB 2139154..2140881 (-) 1728 WP_011002731.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BC350_RS10310 (BC350_10025) - 2141128..2142405 (-) 1278 WP_016723792.1 HlyC/CorC family transporter -
  BC350_RS10320 (BC350_10035) ispB 2142693..2143661 (-) 969 WP_081049965.1 octaprenyl diphosphate synthase -
  BC350_RS10325 (BC350_10040) rplU 2143940..2144251 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  BC350_RS10330 (BC350_10045) rpmA 2144289..2144549 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  BC350_RS10335 (BC350_10050) obgE 2144725..2145825 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63770.60 Da        Isoelectric Point: 7.1546

>NTDB_id=193677 BC350_RS10305 WP_011002731.1 2139154..2140881(-) (pilB) [Ralstonia pseudosolanacearum strain CQPS-1]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=193677 BC350_RS10305 WP_011002731.1 2139154..2140881(-) (pilB) [Ralstonia pseudosolanacearum strain CQPS-1]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCTGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCTTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAAGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAAACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCACTGCCCAAGGAAACGTTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A223GQM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.06

98.435

0.503
  pilF Neisseria gonorrhoeae MS11

52.93

94.957

0.503
  pilB Acinetobacter baylyi ADP1

49.554

97.565

0.483
  pilB Legionella pneumophila strain ERS1305867

47.163

98.087

0.463
  pilB Vibrio cholerae strain A1552

44.821

97.391

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

45.203

94.261

0.426
  pilB Vibrio campbellii strain DS40M4

44.526

95.304

0.424
  pilF Thermus thermophilus HB27

38.256

97.739

0.374

Multiple sequence alignment