Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BEN76_RS15720 Genome accession   NZ_CP016896
Coordinates   3332295..3333053 (+) Length   252 a.a.
NCBI ID   WP_004932917.1    Uniprot ID   -
Organism   Acinetobacter soli strain GFJ2     
Function   assists with secretin assembly and localization (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3327295..3338053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN76_RS15695 (BEN76_15695) - 3328197..3329480 (+) 1284 WP_076033481.1 glycosyltransferase family 4 protein -
  BEN76_RS15700 (BEN76_15700) - 3329506..3330057 (+) 552 WP_004943680.1 phosphatase PAP2 family protein -
  BEN76_RS15705 (BEN76_15705) iscX 3330129..3330329 (+) 201 WP_004932909.1 Fe-S cluster assembly protein IscX -
  BEN76_RS15710 (BEN76_15710) ndk 3330438..3330869 (+) 432 WP_004932912.1 nucleoside-diphosphate kinase -
  BEN76_RS15715 (BEN76_15715) rlmN 3330991..3332235 (+) 1245 WP_004932914.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BEN76_RS15720 (BEN76_15720) pilF 3332295..3333053 (+) 759 WP_004932917.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BEN76_RS15725 (BEN76_15725) - 3333044..3333826 (+) 783 WP_004943670.1 helix-turn-helix domain-containing protein -
  BEN76_RS15730 (BEN76_15730) ispG 3333852..3334967 (+) 1116 WP_004932923.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BEN76_RS15735 (BEN76_15735) hisS 3334964..3336256 (+) 1293 WP_004943667.1 histidine--tRNA ligase -
  BEN76_RS15740 (BEN76_15740) - 3336288..3336989 (+) 702 WP_004932928.1 YfgM family protein -

Sequence

Protein


Download         Length: 252 a.a.        Molecular weight: 28593.56 Da        Isoelectric Point: 9.5007

>NTDB_id=193552 BEN76_RS15720 WP_004932917.1 3332295..3333053(+) (pilF) [Acinetobacter soli strain GFJ2]
MLAALLLSGCQTTSIKKDPQKGAKVRTQLAAEYIKTGDLDAAKRALDHALKVEPRDSNANMMMGVLLQQEGSRPNLEKAD
EYFARAVALDPENAQARNNYGAYLYQMKRYHDAIQQLTRAGSTLGYDQRYLALENLGRIYLHLGGVSNAEKMFKQALQAN
RDSTVSMLELAEIYYLQHKIPASTEMYEQYVRTVGQKNQSARALWVGIRVARANADRTGVQVLVNQLRIFYPDSPEYQRY
MQLQYSTEAVWK

Nucleotide


Download         Length: 759 bp        

>NTDB_id=193552 BEN76_RS15720 WP_004932917.1 3332295..3333053(+) (pilF) [Acinetobacter soli strain GFJ2]
ATGCTGGCGGCGCTTCTTTTATCTGGATGCCAAACGACTTCAATAAAAAAAGATCCGCAAAAAGGCGCGAAAGTCCGGAC
TCAACTTGCAGCCGAATATATCAAAACAGGTGATCTTGATGCTGCCAAGCGTGCGCTAGATCATGCACTCAAAGTCGAAC
CGAGAGACAGCAATGCCAACATGATGATGGGGGTGTTGTTGCAGCAAGAGGGCAGTCGCCCAAATCTTGAAAAAGCCGAT
GAATATTTTGCACGTGCTGTAGCGCTGGATCCGGAGAATGCTCAGGCCAGAAACAACTATGGCGCTTATTTGTATCAGAT
GAAACGTTATCATGATGCAATTCAACAATTGACACGCGCCGGTAGTACATTAGGATATGACCAGCGCTATCTGGCATTGG
AAAATTTAGGTCGGATTTATTTGCATCTGGGGGGTGTTTCGAATGCTGAAAAAATGTTCAAACAGGCACTTCAGGCAAAC
CGTGATTCAACTGTTTCAATGTTAGAGTTAGCAGAAATTTATTATTTGCAACACAAAATTCCTGCCTCGACAGAGATGTA
TGAACAATATGTTCGAACTGTTGGGCAGAAAAATCAGAGTGCTCGTGCACTTTGGGTGGGAATTCGCGTCGCGAGAGCGA
ATGCCGACCGGACTGGGGTGCAAGTATTGGTAAATCAGCTTCGTATTTTTTACCCAGATAGTCCAGAATATCAACGTTAT
ATGCAATTACAGTACAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_10)

(132-161)

Tetratricopeptide repeat (TPR_8)

(23-55)

Tetratricopeptide repeat (TPR_16)

(76-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baylyi ADP1

84.921

100

0.849
  pilF Acinetobacter baumannii D1279779

75.2

99.206

0.746

Multiple sequence alignment