Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BSHJ0_RS13910 Genome accession   NZ_CP016894
Coordinates   2642790..2643200 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain HJ0-6     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2637790..2648200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSHJ0_RS13875 - 2637912..2638052 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  BSHJ0_RS13885 (BSHJ0_02733) yqeF 2638358..2639089 (-) 732 WP_029726702.1 SGNH/GDSL hydrolase family protein -
  BSHJ0_RS13890 (BSHJ0_02734) cwlH 2639341..2640093 (-) 753 WP_029726701.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  BSHJ0_RS13895 (BSHJ0_02735) yqeD 2640280..2640906 (+) 627 WP_038427798.1 TVP38/TMEM64 family protein -
  BSHJ0_RS13900 (BSHJ0_02737) gnd 2640925..2641793 (-) 869 Protein_2691 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  BSHJ0_RS13905 (BSHJ0_02738) yqeB 2642035..2642757 (+) 723 WP_010886572.1 hypothetical protein -
  BSHJ0_RS13910 (BSHJ0_02739) nucA/comI 2642790..2643200 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  BSHJ0_RS13915 (BSHJ0_02740) - 2643396..2643746 (+) 351 Protein_2694 sigma-70 family RNA polymerase sigma factor -
  BSHJ0_RS13920 (BSHJ0_02741) spoIVCA 2643774..2645234 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  BSHJ0_RS23220 - 2645192..2645370 (-) 179 Protein_2696 hypothetical protein -
  BSHJ0_RS13925 (BSHJ0_02742) arsC 2645725..2646144 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  BSHJ0_RS13930 (BSHJ0_02743) acr3 2646156..2647196 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  BSHJ0_RS13935 (BSHJ0_02744) arsK 2647219..2647659 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  BSHJ0_RS13940 (BSHJ0_02745) arsR 2647720..2648037 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=193462 BSHJ0_RS13910 WP_009967785.1 2642790..2643200(-) (nucA/comI) [Bacillus subtilis strain HJ0-6]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=193462 BSHJ0_RS13910 WP_009967785.1 2642790..2643200(-) (nucA/comI) [Bacillus subtilis strain HJ0-6]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAGCAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGATAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACG
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCTGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment