Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BEE12_RS09925 Genome accession   NZ_CP016889
Coordinates   2194888..2196270 (+) Length   460 a.a.
NCBI ID   WP_031593287.1    Uniprot ID   -
Organism   Pantoea agglomerans strain C410P1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2189888..2201270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEE12_RS09900 (BEE12_09900) - 2190951..2191361 (+) 411 WP_022625367.1 DNA polymerase III subunit psi -
  BEE12_RS09905 (BEE12_09905) rimI 2191333..2191773 (+) 441 WP_010253603.1 ribosomal protein S18-alanine N-acetyltransferase -
  BEE12_RS09910 (BEE12_09910) yjjG 2191792..2192472 (+) 681 WP_069025794.1 pyrimidine 5'-nucleotidase -
  BEE12_RS09915 (BEE12_09915) - 2193139..2193789 (-) 651 WP_010253606.1 YtjB family periplasmic protein -
  BEE12_RS09920 (BEE12_09920) serB 2193897..2194874 (+) 978 WP_031593286.1 phosphoserine phosphatase -
  BEE12_RS09925 (BEE12_09925) radA 2194888..2196270 (+) 1383 WP_031593287.1 DNA repair protein RadA Machinery gene
  BEE12_RS09930 (BEE12_09930) nadR 2196346..2197587 (+) 1242 WP_010253612.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  BEE12_RS09935 (BEE12_09935) - 2197633..2198634 (-) 1002 WP_069025795.1 zinc-binding alcohol dehydrogenase family protein -
  BEE12_RS09940 (BEE12_09940) - 2198839..2199735 (+) 897 WP_010253616.1 LysR family transcriptional regulator -
  BEE12_RS09945 (BEE12_09945) - 2199716..2200318 (-) 603 WP_069025796.1 putative adenosine monophosphate-protein transferase Fic -
  BEE12_RS24880 - 2200331..2200495 (-) 165 WP_109652447.1 YhfG family protein -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49307.93 Da        Isoelectric Point: 7.4220

>NTDB_id=193381 BEE12_RS09925 WP_031593287.1 2194888..2196270(+) (radA) [Pantoea agglomerans strain C410P1]
MAKAAKRAFVCNECGADYPRWQGQCSACHAWNTITEVRIAASPAAARNERLSGYAGSAGTSRVQKLSEISLEALPRFSTS
FKEFDRVLGGGVVPGSAILIGGSPGAGKSTLLLQVMCRLSEGMKTLYVTGEESLQQVAMRAHRLGLPTENVNMLSETSIE
QICLIAEQEQPQLMVIDSIQVMHMAEIQSSPGSVAQVRETAAYLTRFAKTRGVAIIMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGMREVSNPSAIFLSRGEEVTSGSSVMVLWEGTRPLLVEIQALVDH
SMMGNPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALMLSMVSSLRDRPLPQDLVIFGEV
GLAGEIRPVPSGQERIAEAAKHGFKRAIVPAGNAPKKPIAGMQVFSAKKLADALAILDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=193381 BEE12_RS09925 WP_031593287.1 2194888..2196270(+) (radA) [Pantoea agglomerans strain C410P1]
TTGGCCAAAGCGGCAAAACGCGCATTTGTCTGTAACGAATGTGGCGCAGATTACCCACGCTGGCAGGGGCAGTGCAGCGC
CTGTCACGCCTGGAACACCATCACTGAGGTGCGCATTGCCGCCTCGCCCGCGGCTGCGCGTAACGAGCGCCTGAGTGGCT
ATGCCGGCAGCGCCGGAACCAGCCGGGTGCAGAAGCTCTCTGAAATCAGTTTAGAGGCGTTACCGCGCTTCTCTACCAGC
TTCAAAGAGTTTGACCGGGTGCTGGGCGGCGGGGTGGTGCCGGGCAGCGCCATTCTGATTGGCGGCAGCCCCGGCGCAGG
CAAATCCACGCTGCTGCTGCAGGTGATGTGCCGGTTGTCAGAGGGCATGAAAACCCTGTATGTCACCGGTGAAGAGTCAC
TACAACAGGTGGCGATGCGCGCCCATCGTCTGGGATTACCTACCGAAAATGTGAATATGCTGTCGGAAACCAGCATTGAG
CAGATCTGCCTGATCGCCGAACAGGAGCAGCCGCAACTGATGGTGATCGACTCGATCCAGGTGATGCACATGGCGGAGAT
CCAGTCATCGCCTGGCAGCGTCGCCCAGGTGCGTGAAACCGCCGCTTACCTGACGCGCTTCGCCAAGACGCGCGGTGTGG
CGATCATCATGGTCGGTCACGTCACCAAAGATGGCTCCCTGGCCGGACCGAAAGTACTGGAGCACTGTATCGACTGTTCG
GTACTGCTGGATGGCGATGCCGATTCCCGCTTCCGCACCCTGCGCAGTCATAAAAACCGCTTCGGCGCGGTCAATGAACT
GGGTGTGTTTGCCATGACCGAGCAGGGCATGCGTGAAGTCAGCAACCCGTCAGCTATTTTCCTGTCGCGCGGCGAAGAGG
TCACCTCGGGCAGTTCGGTGATGGTGCTGTGGGAAGGAACGCGACCGCTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGGCAATCCACGCCGCGTGGCAGTGGGTCTGGAGCAGAACCGTCTGGCGATTCTGCTGGCGGTGCTGCACCG
GCACGGCGGATTGCAGATGGCGGATCAGGATGTGTTCGTTAACGTGGTCGGCGGCGTCAAAGTCACCGAAACCAGTGCAG
ACCTGGCGCTGATGCTGTCGATGGTGTCGAGCCTGCGCGATCGTCCGTTGCCGCAGGATCTGGTGATCTTCGGCGAAGTG
GGACTGGCGGGCGAAATCCGTCCGGTGCCCAGCGGTCAGGAGCGCATCGCTGAAGCGGCGAAGCATGGCTTCAAACGCGC
TATTGTACCGGCAGGGAATGCACCGAAAAAACCGATTGCCGGGATGCAGGTTTTCAGCGCGAAGAAGCTGGCCGATGCGC
TGGCGATTCTGGACGATCTGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(347-433)

AAA domain (AAA_25)

(87-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.373

100

0.485
  radA Streptococcus mitis NCTC 12261

44.82

96.522

0.433
  radA Streptococcus pneumoniae Rx1

45.244

93.696

0.424
  radA Streptococcus pneumoniae D39

45.244

93.696

0.424
  radA Streptococcus pneumoniae R6

45.244

93.696

0.424
  radA Streptococcus pneumoniae TIGR4

45.244

93.696

0.424
  radA Streptococcus mitis SK321

45.244

93.696

0.424

Multiple sequence alignment