Detailed information NTG0193201

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BE845_RS03625
• Genome accession: NZ_CP016874
• Coordinates: 803781..804845 (-)
• Length: 354 a.a.
• NCBI ID: WP_011215094.1
• Uniprot ID: Q5WXY1
• Organism: Legionella pneumophila subsp. pneumophila strain FFI329
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 798781..809845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BE845_RS03600 (BE845_03600)	aroK	798962..799489 (-)	528	WP_010946667.1	shikimate kinase AroK	-
BE845_RS03605 (BE845_03605)	pilQ	799935..802031 (-)	2097	WP_070322471.1	type IV pilus secretin PilQ	Machinery gene
BE845_RS03610 (BE845_03610)	pilP	802035..802619 (-)	585	WP_011215097.1	pilus assembly protein PilP	Machinery gene
BE845_RS03615 (BE845_03615)	pilO	802616..803215 (-)	600	WP_011215096.1	type 4a pilus biogenesis protein PilO	Machinery gene
BE845_RS03620 (BE845_03620)	pilN	803229..803777 (-)	549	WP_011215095.1	PilN domain-containing protein	Machinery gene
BE845_RS03625 (BE845_03625)	pilM	803781..804845 (-)	1065	WP_011215094.1	type IV pilus assembly protein PilM	Machinery gene
BE845_RS03630 (BE845_03630)	ravI	805262..806269 (+)	1008	WP_011215093.1	Dot/Icm T4SS effector RavI	-
BE845_RS03635 (BE845_03635)	-	806421..808805 (+)	2385	WP_011215092.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38760.78 Da
• Isoelectric Point: 5.0367

>NTDB_id=193201 BE845_RS03625 WP_011215094.1 803781..804845(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI329]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=193201 BE845_RS03625 WP_011215094.1 803781..804845(-) (pilM) [Legionella pneumophila subsp. pneumophila strain FFI329]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAAGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTGGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTATCCCATAGATGAAATAAATCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
GGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACCCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCGATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGATAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCATGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5WXY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	100	100	1
comM	Acinetobacter nosocomialis M2	42.373	100	0.424
pilM	Acinetobacter baumannii D1279779	42.09	100	0.421
comM	Acinetobacter baylyi ADP1	40.678	100	0.407