Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BFI33_RS14020 Genome accession   NZ_CP016852
Coordinates   2651759..2652169 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain 168G     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2646759..2657169
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFI33_RS13985 (BFI33_13985) - 2646846..2646986 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  BFI33_RS13995 (BFI33_13995) yqeF 2647292..2648023 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  BFI33_RS14000 (BFI33_14000) cwlH 2648275..2649027 (-) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  BFI33_RS14005 (BFI33_14005) yqeD 2649214..2649840 (+) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  BFI33_RS14010 (BFI33_14010) gnd 2649859..2650752 (-) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  BFI33_RS14015 (BFI33_14015) yqeB 2651004..2651726 (+) 723 WP_010886572.1 hypothetical protein -
  BFI33_RS14020 (BFI33_14020) nucA/comI 2651759..2652169 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  BFI33_RS14025 (BFI33_14025) - 2652365..2652715 (+) 351 Protein_2700 sigma-70 family RNA polymerase sigma factor -
  BFI33_RS14030 (BFI33_14030) spoIVCA 2652743..2654203 (-) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  BFI33_RS23160 - 2654161..2654339 (-) 179 Protein_2702 hypothetical protein -
  BFI33_RS14035 (BFI33_14035) arsC 2654694..2655113 (-) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  BFI33_RS14040 (BFI33_14040) acr3 2655125..2656165 (-) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  BFI33_RS14045 (BFI33_14045) arsK 2656188..2656628 (-) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  BFI33_RS14050 (BFI33_14050) arsR 2656689..2657006 (-) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=192789 BFI33_RS14020 WP_009967785.1 2651759..2652169(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain 168G]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=192789 BFI33_RS14020 WP_009967785.1 2651759..2652169(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain 168G]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment