Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BFI33_RS00590 Genome accession   NZ_CP016852
Coordinates   106090..107466 (+) Length   458 a.a.
NCBI ID   WP_004399687.1    Uniprot ID   A0AAE2SEF1
Organism   Bacillus subtilis subsp. subtilis strain 168G     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101090..112466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFI33_RS00570 (BFI33_00570) ctsR 101443..101907 (+) 465 WP_003225724.1 transcriptional regulator CtsR -
  BFI33_RS00575 (BFI33_00575) mcsA 101921..102478 (+) 558 WP_009966297.1 protein-arginine kinase activator protein McsA -
  BFI33_RS00580 (BFI33_00580) mcsB 102478..103569 (+) 1092 WP_003235007.1 protein arginine kinase -
  BFI33_RS00585 (BFI33_00585) clpC 103566..105998 (+) 2433 WP_003235011.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BFI33_RS00590 (BFI33_00590) radA 106090..107466 (+) 1377 WP_004399687.1 DNA repair protein RadA Machinery gene
  BFI33_RS00595 (BFI33_00595) disA 107470..108552 (+) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  BFI33_RS00600 (BFI33_00600) yacL 108668..109768 (+) 1101 WP_003235014.1 PIN/TRAM domain-containing protein -
  BFI33_RS00605 (BFI33_00605) ispD 109783..110481 (+) 699 WP_003235019.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BFI33_RS00610 (BFI33_00610) ispF 110474..110950 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49482.99 Da        Isoelectric Point: 8.2399

>NTDB_id=192736 BFI33_RS00590 WP_004399687.1 106090..107466(+) (radA) [Bacillus subtilis subsp. subtilis strain 168G]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIVISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=192736 BFI33_RS00590 WP_004399687.1 106090..107466(+) (radA) [Bacillus subtilis subsp. subtilis strain 168G]
ATGGCTAAAACAAAATCGAAGTTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
TGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCATCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCTGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCTACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCACTCATCTCGCCAACAAGTTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCGA
TTGTGATTAGTATTGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

62.914

98.908

0.622

Multiple sequence alignment