Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BFC19_RS06665 Genome accession   NZ_CP016839
Coordinates   1355385..1356770 (-) Length   461 a.a.
NCBI ID   WP_029091568.1    Uniprot ID   A0A1D2KGY7
Organism   Brochothrix thermosphacta strain TMW 2.1564     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1350385..1361770
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BFC19_RS06640 (BFC19_06590) epsC 1350636..1351274 (-) 639 WP_069129622.1 serine O-acetyltransferase EpsC -
  BFC19_RS06645 (BFC19_06595) gltX 1351591..1353060 (-) 1470 WP_069118452.1 glutamate--tRNA ligase -
  BFC19_RS06650 (BFC19_06600) ispF 1353096..1353569 (-) 474 WP_119946276.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BFC19_RS06655 (BFC19_06605) ispD 1353575..1354273 (-) 699 WP_119945763.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BFC19_RS06660 (BFC19_06610) - 1354287..1355369 (-) 1083 WP_029091567.1 PIN/TRAM domain-containing protein -
  BFC19_RS06665 (BFC19_06615) radA 1355385..1356770 (-) 1386 WP_029091568.1 DNA repair protein RadA Machinery gene
  BFC19_RS06670 (BFC19_06620) - 1356977..1359478 (-) 2502 WP_119945764.1 ATP-dependent Clp protease ATP-binding subunit -
  BFC19_RS06675 (BFC19_06625) - 1359509..1359967 (-) 459 WP_029091570.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50638.30 Da        Isoelectric Point: 6.4051

>NTDB_id=192597 BFC19_RS06665 WP_029091568.1 1355385..1356770(-) (radA) [Brochothrix thermosphacta strain TMW 2.1564]
MAKKKASTKFVCNACGYESPKWMGRCPNCSEWNQMVEFVEAKVQKGRTSFNHTEEMESQQALPLSKITSETEQRILTDMP
ELNRVLGGGLVKGSLVLVGGDPGIGKSTLLLQVSAQLTTAKRKGLYVSGEESIKQTKLRAERLNVDGDLLYVYAQTDLEV
ISKTIQTLQPEFVVIDSIQTIYHPDVTSAAGSVTQVRECTSELMRLAKTLNIAIFIVGHVTKEGTIAGPRMLEHMVDTVL
YFEGERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVDNASEVFLEERLEGAAGSAVVTSMEGTRPVLVEIQALVSPT
VYGNARRMATGMDYNRVSLIMAVLEKREGLMLQNQDAYLKAAGGVKLDEPAIDLAIAISIASSYYDTPTAAMDCFIGEVG
LTGELRRVARIDQRVQEAAKLGFKRIIIPANNIGGWTFPAEIEVVGVSTLKDAIHKAFPNR

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=192597 BFC19_RS06665 WP_029091568.1 1355385..1356770(-) (radA) [Brochothrix thermosphacta strain TMW 2.1564]
ATGGCAAAGAAAAAAGCGAGTACTAAATTTGTCTGCAACGCCTGCGGATATGAGTCTCCAAAATGGATGGGGCGTTGCCC
TAACTGTAGCGAATGGAACCAAATGGTCGAATTTGTTGAGGCAAAAGTCCAAAAAGGACGAACTAGTTTTAATCATACAG
AAGAAATGGAAAGTCAGCAGGCATTGCCTCTTTCTAAAATAACAAGTGAAACTGAACAACGTATCTTAACAGATATGCCA
GAATTGAATCGTGTCCTCGGCGGGGGATTAGTTAAAGGTTCATTGGTGTTGGTCGGTGGTGACCCAGGGATTGGTAAATC
AACACTATTGTTGCAAGTATCAGCACAACTCACAACAGCAAAACGCAAAGGCCTTTATGTTTCGGGAGAAGAATCAATCA
AGCAAACAAAACTACGGGCGGAACGTTTAAATGTGGATGGGGATTTATTATATGTTTATGCGCAAACCGACTTAGAAGTC
ATCTCTAAAACAATTCAGACTTTACAGCCGGAATTTGTCGTGATTGATTCGATCCAAACCATCTATCACCCTGATGTGAC
AAGTGCAGCAGGGAGCGTCACACAAGTAAGAGAGTGTACGTCAGAATTGATGCGTCTTGCAAAGACGTTAAACATTGCCA
TCTTCATTGTCGGTCATGTGACAAAAGAGGGTACAATTGCAGGTCCACGTATGTTAGAACATATGGTTGACACTGTTTTA
TACTTTGAAGGTGAACGCCACCATGCCTACCGGATTTTACGCGCCGTAAAAAATAGGTTTGGATCTACCAATGAAATGGG
TATCTTTGAAATGCGCGATGTCGGTTTGGTAGAGGTCGACAACGCCTCAGAAGTATTTTTAGAAGAGCGTTTAGAAGGAG
CTGCTGGTTCTGCAGTTGTTACCTCAATGGAGGGGACACGTCCTGTTCTGGTAGAGATTCAAGCATTGGTGTCACCGACT
GTTTATGGAAATGCACGTCGAATGGCAACAGGAATGGATTATAATCGTGTTTCTCTAATTATGGCGGTACTTGAGAAACG
TGAAGGTTTAATGCTTCAAAATCAAGATGCCTATCTTAAAGCAGCAGGTGGTGTGAAGTTAGACGAGCCTGCAATTGATT
TAGCAATAGCAATAAGTATTGCATCGAGTTATTACGATACACCAACAGCAGCAATGGATTGCTTTATTGGTGAAGTCGGT
TTAACGGGAGAGTTACGTCGTGTTGCTCGTATTGATCAACGCGTACAAGAAGCTGCAAAACTAGGTTTCAAACGTATTAT
TATACCAGCGAACAATATCGGTGGTTGGACATTCCCTGCTGAGATTGAAGTAGTAGGTGTATCAACATTAAAAGATGCGA
TTCATAAAGCATTTCCAAATAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

AAA domain (AAA_25)

(66-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D2KGY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.756

99.566

0.675
  radA Streptococcus pneumoniae Rx1

64.13

99.783

0.64
  radA Streptococcus pneumoniae D39

64.13

99.783

0.64
  radA Streptococcus pneumoniae R6

64.13

99.783

0.64
  radA Streptococcus pneumoniae TIGR4

64.13

99.783

0.64
  radA Streptococcus mitis SK321

64.13

99.783

0.64
  radA Streptococcus mitis NCTC 12261

64.13

99.783

0.64

Multiple sequence alignment