Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BC359_RS00445 Genome accession   NZ_CP016790
Coordinates   71505..72884 (-) Length   459 a.a.
NCBI ID   WP_078988978.1    Uniprot ID   -
Organism   Priestia flexa strain KLBMP 4941     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 66505..77884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BC359_RS00425 (BC359_00425) ispF 67976..68455 (-) 480 WP_025909703.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BC359_RS00430 (BC359_00430) ispD 68468..69157 (-) 690 WP_078988976.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BC359_RS00435 (BC359_00435) - 69183..70280 (-) 1098 WP_061784782.1 PIN/TRAM domain-containing protein -
  BC359_RS00440 (BC359_00440) disA 70423..71499 (-) 1077 WP_078988977.1 DNA integrity scanning diadenylate cyclase DisA -
  BC359_RS00445 (BC359_00445) radA 71505..72884 (-) 1380 WP_078988978.1 DNA repair protein RadA Machinery gene
  BC359_RS00450 (BC359_00450) clpC 73008..75449 (-) 2442 WP_061784784.1 ATP-dependent protease ATP-binding subunit ClpC -
  BC359_RS00455 (BC359_00455) - 75470..76537 (-) 1068 WP_025909697.1 protein arginine kinase -
  BC359_RS00460 (BC359_00460) - 76542..77081 (-) 540 WP_025909696.1 UvrB/UvrC motif-containing protein -
  BC359_RS00465 (BC359_00465) - 77119..77580 (-) 462 WP_078988979.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49699.90 Da        Isoelectric Point: 7.4549

>NTDB_id=192239 BC359_RS00445 WP_078988978.1 71505..72884(-) (radA) [Priestia flexa strain KLBMP 4941]
MSKRKTKFSCQHCGYESAKWMGKCPGCGSWNSMVEEIEETKSSRRGGAFSGATSTKVQKPQSITNIESTTEPRIYTKSSE
LNRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMNKHKVLYISGEESTKQTKLRADRLGIKAEDLYVHAETNLESI
TEAISSMNPELVIIDSIQTIYHGDVTSAPGSVSQVRECTAELMRIAKTNGIAIFIVGHVTKEGSIAGPRLLEHMVDSVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTS
FGNPRRMSTGLDHNRVSLIMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFKDAPSNPTDVVVGEVGL
TGEVRRVSRIEQRVQEAVKLGFKRIIVPEKNLGGWKIPEGIDVVGVSTVSEALQYTLGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=192239 BC359_RS00445 WP_078988978.1 71505..72884(-) (radA) [Priestia flexa strain KLBMP 4941]
ATGAGTAAAAGAAAAACAAAGTTTTCATGTCAACATTGTGGGTATGAATCGGCTAAATGGATGGGAAAGTGTCCTGGCTG
TGGCAGCTGGAATTCAATGGTGGAAGAAATTGAAGAGACAAAGTCTTCAAGGCGAGGAGGGGCTTTTTCTGGCGCAACGT
CTACAAAGGTGCAAAAGCCTCAATCCATTACGAATATTGAATCAACGACTGAACCAAGGATATATACAAAATCATCCGAG
CTAAACCGTGTTTTAGGTGGAGGGATTGTTAGAGGATCACTAGTCTTAATTGGGGGAGATCCTGGTATTGGTAAATCAAC
TTTGTTGCTACAAACTTCAGCTCAGTTAGCTATGAACAAGCATAAGGTGCTTTATATATCTGGTGAGGAATCAACAAAGC
AAACAAAGCTAAGAGCAGATCGCTTAGGTATTAAAGCGGAAGATTTGTATGTGCATGCAGAAACAAACTTAGAGTCAATC
ACAGAAGCTATCTCAAGTATGAATCCAGAACTTGTTATCATTGATTCAATTCAAACAATTTATCACGGTGACGTTACATC
AGCGCCCGGAAGTGTTTCTCAAGTACGCGAGTGTACAGCAGAGTTAATGCGAATTGCAAAAACAAACGGCATAGCTATTT
TTATTGTAGGTCATGTTACCAAAGAAGGGTCTATTGCAGGTCCAAGGTTACTTGAACATATGGTAGACAGTGTTTTGTAT
TTTGAAGGAGAACGCCACCATACGTACCGGATTTTAAGAGCAGTAAAAAACCGCTTTGGTTCAACAAACGAAATGGGCAT
TTTTGAAATGAAGGAAAGTGGTCTAGAGGAAGTGCTCAACCCATCTGAAATCTTTTTAGAAGAACGATCTCAAGGTGCAG
CTGGTTCTGTCGTTGTGGCTTCGATGGAGGGTACTCGTCCCGTATTGGTAGAGTTACAAGCACTTATTAGCCCAACAAGC
TTTGGAAATCCTAGAAGAATGTCTACAGGACTTGATCATAACCGTGTCTCGCTTATTATGGCAGTGTTAGAAAAGCGAGT
AGGATTACTGTTGCAAAATCAAGATGCGTACTTAAAAGTAGCAGGTGGGGTAAAGTTAGATGAGCCAGCTATTGATTTGG
CGGTTGCGGTTAGCATTGCATCGAGTTTTAAAGATGCACCTTCAAACCCTACAGACGTAGTAGTAGGAGAAGTTGGATTA
ACAGGTGAGGTTCGAAGAGTGTCTCGCATTGAACAAAGAGTGCAAGAAGCTGTGAAATTAGGGTTTAAGCGAATCATTGT
TCCTGAAAAAAACTTAGGTGGTTGGAAAATACCAGAAGGCATTGACGTAGTAGGGGTGTCCACTGTGTCAGAAGCGTTAC
AGTACACGTTAGGAGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-431)

AAA domain (AAA_25)

(69-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.381

100

0.754
  radA Streptococcus pneumoniae Rx1

63.216

98.911

0.625
  radA Streptococcus pneumoniae D39

63.216

98.911

0.625
  radA Streptococcus pneumoniae R6

63.216

98.911

0.625
  radA Streptococcus pneumoniae TIGR4

63.216

98.911

0.625
  radA Streptococcus mitis NCTC 12261

63.216

98.911

0.625
  radA Streptococcus mitis SK321

63.216

98.911

0.625

Multiple sequence alignment