Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BEN31_RS04665 Genome accession   NZ_CP016784
Coordinates   877150..877920 (+) Length   256 a.a.
NCBI ID   WP_034661344.1    Uniprot ID   -
Organism   Bacillus pumilus strain PDSLzg-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 872150..882920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN31_RS04635 (BEN31_04635) - 872457..874568 (+) 2112 WP_411170939.1 HD family phosphohydrolase -
  BEN31_RS04640 (BEN31_04640) ybeY 874569..875048 (+) 480 WP_012010615.1 rRNA maturation RNase YbeY -
  BEN31_RS04645 (BEN31_04645) - 875026..875412 (+) 387 WP_066030644.1 diacylglycerol kinase family protein -
  BEN31_RS04650 (BEN31_04650) - 875526..875924 (+) 399 WP_034661347.1 cytidine deaminase -
  BEN31_RS04655 (BEN31_04655) era 875917..876822 (+) 906 WP_060596663.1 GTPase Era -
  BEN31_RS04660 (BEN31_04660) - 876971..877114 (+) 144 WP_003217288.1 YqzL family protein -
  BEN31_RS04665 (BEN31_04665) recO 877150..877920 (+) 771 WP_034661344.1 DNA repair protein RecO Machinery gene
  BEN31_RS04670 (BEN31_04670) glyQ 878253..879140 (+) 888 WP_012010611.1 glycine--tRNA ligase subunit alpha -
  BEN31_RS04675 (BEN31_04675) glyS 879133..881178 (+) 2046 WP_066030645.1 glycine--tRNA ligase subunit beta -
  BEN31_RS04680 (BEN31_04680) - 881345..881983 (+) 639 WP_024423017.1 helix-turn-helix transcriptional regulator -
  BEN31_RS04685 (BEN31_04685) - 882009..882830 (+) 822 WP_008342399.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 256 a.a.        Molecular weight: 29244.92 Da        Isoelectric Point: 8.4348

>NTDB_id=192168 BEN31_RS04665 WP_034661344.1 877150..877920(+) (recO) [Bacillus pumilus strain PDSLzg-1]
MLIKSEGIVLRTTDYGETNKIVTLLTREHGKIGVMARGAKKSNSRLSAISQPFLYGTFLIQSSTGLGTLQQGEMIESMRT
IREDLFLTAYAAYMTELLDKGTEEKKPNPYLFELLLQSLRHLNEGTDADIILFIVEVKMLSVMGMKPELDQCVHCGQKEG
QFHFSIRDNGFICQNCFSKDPYKLPLSPAAARLLRLFHYFDLSRLGQVDVKPQTKQEIRQVLDHYYEEYSGLYLKSKKFM
NQMESMKKLMGGENKS

Nucleotide


Download         Length: 771 bp        

>NTDB_id=192168 BEN31_RS04665 WP_034661344.1 877150..877920(+) (recO) [Bacillus pumilus strain PDSLzg-1]
ATGCTCATTAAAAGTGAAGGAATCGTCCTTAGAACAACAGATTATGGCGAAACAAACAAAATTGTCACGCTGCTCACTAG
AGAACATGGGAAAATTGGCGTGATGGCAAGAGGTGCAAAAAAATCAAACAGCCGCCTGTCAGCCATAAGCCAGCCGTTTT
TATACGGAACCTTTCTGATCCAATCATCAACAGGACTTGGAACGCTCCAGCAAGGCGAAATGATTGAAAGTATGCGAACC
ATTCGGGAGGATTTATTTTTGACCGCATATGCCGCATATATGACAGAGTTACTCGATAAAGGCACAGAAGAAAAAAAGCC
AAACCCTTATTTGTTTGAGCTCCTGCTTCAATCTCTGCGTCATCTGAATGAAGGCACAGATGCTGATATTATCTTATTTA
TCGTAGAGGTCAAAATGTTATCAGTAATGGGAATGAAGCCTGAACTTGATCAATGTGTCCATTGTGGTCAAAAAGAAGGG
CAGTTTCATTTCTCTATTAGAGATAACGGCTTCATTTGCCAAAATTGCTTTTCTAAAGATCCGTATAAACTGCCCTTATC
CCCAGCAGCTGCAAGGCTGCTCCGTTTATTTCATTATTTTGATTTATCAAGGCTCGGTCAGGTTGATGTGAAACCGCAGA
CGAAACAAGAAATTCGTCAAGTGCTCGATCACTACTATGAAGAGTATTCAGGCCTTTATTTGAAATCAAAAAAATTCATG
AATCAGATGGAATCCATGAAAAAGCTAATGGGCGGGGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

75.781

100

0.758

Multiple sequence alignment