Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   CA935_RS14945 Genome accession   NZ_CP021258
Coordinates   3325945..3328122 (+) Length   725 a.a.
NCBI ID   WP_027228734.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. fraseri strain D-5744     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3320945..3333122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA935_RS14915 - 3321417..3321860 (-) 444 WP_027228739.1 hypothetical protein -
  CA935_RS14920 - 3321942..3322928 (+) 987 WP_027228738.1 tRNA-dihydrouridine synthase family protein -
  CA935_RS14925 - 3322987..3323346 (-) 360 WP_027228737.1 hypothetical protein -
  CA935_RS14930 - 3323403..3324158 (-) 756 WP_027228736.1 pteridine reductase -
  CA935_RS14935 - 3324335..3325447 (+) 1113 WP_027228735.1 SAM-dependent methyltransferase -
  CA935_RS14940 - 3325450..3325944 (+) 495 WP_011216699.1 6-carboxytetrahydropterin synthase -
  CA935_RS14945 priA 3325945..3328122 (+) 2178 WP_027228734.1 primosomal protein N' Machinery gene
  CA935_RS14950 - 3328119..3328511 (+) 393 WP_027228733.1 thioesterase family protein -
  CA935_RS14955 - 3328621..3328890 (-) 270 WP_027228732.1 hypothetical protein -
  CA935_RS14960 thyA 3328966..3329760 (-) 795 WP_027228731.1 thymidylate synthase -
  CA935_RS14965 lgt 3329757..3330527 (-) 771 WP_027228730.1 prolipoprotein diacylglyceryl transferase -
  CA935_RS14970 - 3330545..3331351 (-) 807 WP_027228729.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 725 a.a.        Molecular weight: 81553.78 Da        Isoelectric Point: 8.8584

>NTDB_id=191433 CA935_RS14945 WP_027228734.1 3325945..3328122(+) (priA) [Legionella pneumophila subsp. fraseri strain D-5744]
MNDIYQVCIPHTNRDCFDYEALDLTPCIGGRVWVPFRNQTRVGIVINKICSQQTDHSLKQIISIIDEKPLIPDALLELCL
WVGSYYQSPLSEVIPLVIPKKYRQGLPCSLPTEDVYQLNLSAENAKTLISKKAKKQHELIDFLSRHQDGVSKQTLKQHGY
NGAQIAALLACQAINLSQQVTLPTKIPEKLSPPLTLNPEQAVAVASILEHIHHYQCFLLQGVTGSGKTEVYLHVIAKILE
QGKQILVLVPEIGLTPQLLSRFTARFDQPIAVLHSNLNESERQIAWQLAKENKVKMVIGTRSAVFTPLPNLGLIIIDEEH
DSSLKQMEGVRYSARDTALMRAHLANIPIILGTATPSLETIHNCKQKKYNLLRLTHKAASTTPLHYQLIDLRSQMVQHGL
ALPTLKIIENHLLQQNQVLVFINRRGFAPVLLCHQCGWMVDCKACDSHLTLHKQAGQMICHHCGLTQRIPLFCNNCQSKE
LIPVGSGTQRIYEFLSHQFPDTNVLRIDRDEVRKKNSLNSHLDKINKGEAQLIIGTQMLAKGHHFPRLSLVVVVDADAGF
YNQDFRAIEHLGQLLTQVSGRAGRAEHPGQVLIQTYLPDHPLLNLLIQHGYDEFANALLISRQEAEMPPFQFLAVIRAQG
KTVNKVDQFLKATKDQIQIHPLTVLGPAPAPLPRKANQHRMQLLIKSPSRKVLKSSLTQLREWLTMNKLSNGIRWNVDVD
PMDLS

Nucleotide


Download         Length: 2178 bp        

>NTDB_id=191433 CA935_RS14945 WP_027228734.1 3325945..3328122(+) (priA) [Legionella pneumophila subsp. fraseri strain D-5744]
ATGAACGATATTTATCAAGTTTGTATCCCCCATACCAATCGAGATTGTTTTGATTACGAAGCCCTGGATTTAACTCCATG
CATTGGCGGAAGAGTATGGGTTCCTTTCCGCAATCAAACCCGAGTGGGCATTGTAATCAATAAAATCTGTAGCCAACAAA
CGGACCATTCGTTAAAGCAAATCATTTCAATAATCGATGAGAAACCTCTAATTCCTGATGCTCTCTTAGAGCTTTGTCTA
TGGGTAGGAAGCTATTATCAGTCACCATTATCTGAAGTCATTCCTTTGGTAATACCCAAAAAATATCGTCAGGGTTTACC
CTGCTCGCTACCTACAGAAGATGTTTATCAATTGAATCTCTCTGCTGAAAATGCTAAAACATTAATCTCGAAAAAAGCAA
AAAAACAACATGAATTGATTGATTTTTTAAGCAGGCACCAGGATGGCGTTTCCAAACAGACCCTAAAACAGCATGGCTAC
AACGGAGCTCAAATTGCGGCACTACTTGCTTGTCAAGCCATTAACCTGTCACAGCAAGTGACTTTGCCAACCAAAATTCC
TGAAAAACTCAGCCCACCATTAACATTAAATCCTGAACAAGCCGTTGCCGTAGCTTCCATTTTAGAACATATCCATCATT
ACCAATGCTTTTTACTGCAAGGCGTTACCGGAAGTGGTAAAACAGAAGTTTATTTGCATGTCATCGCCAAAATATTGGAG
CAAGGCAAACAAATATTGGTGTTGGTACCTGAAATCGGGTTAACCCCTCAGCTTTTATCCCGTTTTACAGCCCGCTTTGA
TCAGCCCATAGCCGTATTACATTCCAATTTGAATGAATCAGAAAGGCAAATTGCCTGGCAGCTGGCAAAAGAAAACAAAG
TTAAAATGGTGATTGGAACTCGTTCTGCCGTTTTTACCCCTCTGCCCAATCTCGGTTTAATTATCATCGATGAAGAGCAT
GACAGTTCGCTCAAACAAATGGAAGGAGTGCGTTATTCTGCGCGTGATACTGCTTTAATGCGTGCTCATCTTGCCAATAT
TCCTATCATTCTTGGTACAGCCACTCCCAGTCTGGAAACCATCCACAACTGCAAACAAAAAAAATATAATTTATTACGCT
TAACGCATAAAGCTGCCTCCACTACACCATTACACTATCAACTGATTGATTTACGATCGCAAATGGTGCAGCATGGCTTG
GCTCTCCCAACCCTTAAAATCATAGAAAATCATTTGCTGCAGCAAAACCAGGTACTTGTTTTCATTAATCGACGTGGCTT
TGCCCCTGTCTTACTTTGCCATCAGTGCGGATGGATGGTTGATTGCAAAGCATGCGATAGTCATTTAACCCTGCATAAAC
AAGCAGGACAAATGATTTGTCATCATTGCGGATTAACCCAAAGAATACCCTTGTTTTGCAATAACTGCCAAAGCAAGGAA
TTAATCCCCGTAGGATCTGGCACCCAGAGAATTTATGAGTTTTTAAGCCATCAGTTTCCAGATACGAATGTTTTACGAAT
TGACCGAGATGAGGTTCGTAAAAAAAATTCTCTAAACAGCCATTTGGACAAAATTAATAAAGGGGAAGCTCAGCTGATCA
TAGGCACTCAAATGCTAGCCAAGGGACACCATTTCCCCCGCCTATCCCTGGTCGTTGTCGTAGATGCTGATGCAGGCTTT
TACAACCAGGATTTCCGGGCTATTGAACATTTGGGGCAATTATTAACTCAAGTATCTGGACGAGCAGGGCGAGCAGAACA
TCCCGGGCAAGTATTGATTCAAACTTATTTACCTGATCATCCCCTGCTTAATCTACTCATTCAGCATGGGTATGATGAAT
TTGCGAACGCTTTATTAATTTCAAGACAAGAGGCTGAAATGCCACCTTTTCAGTTTTTAGCGGTCATCAGAGCTCAGGGC
AAAACCGTCAACAAGGTGGATCAATTCCTAAAAGCAACCAAAGATCAAATTCAGATTCACCCTCTCACTGTACTGGGTCC
AGCACCAGCTCCTTTGCCACGAAAAGCTAACCAGCATAGGATGCAACTATTAATTAAATCCCCTTCAAGAAAAGTTCTAA
AAAGTTCATTGACGCAATTAAGAGAGTGGTTAACAATGAACAAATTAAGTAATGGCATCCGCTGGAATGTGGATGTTGAT
CCTATGGACTTATCATGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(442-468)

Type III restriction enzyme, res subunit (ResIII)

(195-357)

3DNA-binding domain (3BD) (PriA_3primeBD)

(6-99)

Primosomal protein N C-terminal domain (PriA_C)

(629-721)

Helicase conserved C-terminal domain (Helicase_C)

(489-585)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

41.45

100

0.418