Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   B9W17_RS21800 Genome accession   NZ_CP020933
Coordinates   4450708..4451148 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain HB-Coli0     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4445708..4456148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9W17_RS21780 (B9W17_22935) aroP 4446706..4448079 (+) 1374 WP_000969914.1 aromatic amino acid transporter AroP -
  B9W17_RS21785 (B9W17_22940) ampE 4448122..4448976 (-) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  B9W17_RS21790 (B9W17_22945) ampD 4448973..4449524 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  B9W17_RS21795 (B9W17_22950) nadC 4449612..4450505 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  B9W17_RS21800 (B9W17_22960) pilA 4450708..4451148 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  B9W17_RS21805 (B9W17_22965) pilB 4451158..4452543 (+) 1386 WP_001470984.1 type II secretion system protein GspE Machinery gene
  B9W17_RS21810 (B9W17_22970) hofC 4452533..4453735 (+) 1203 WP_000157234.1 protein transport protein HofC -
  B9W17_RS21815 (B9W17_22975) guaC 4453770..4454813 (-) 1044 WP_001217338.1 GMP reductase -
  B9W17_RS25365 - 4454969..4455013 (-) 45 WP_120795372.1 protein YacM -
  B9W17_RS21820 (B9W17_22980) coaE 4455038..4455658 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=189966 B9W17_RS21800 WP_000360895.1 4450708..4451148(+) (pilA) [Escherichia coli strain HB-Coli0]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=189966 B9W17_RS21800 WP_000360895.1 4450708..4451148(+) (pilA) [Escherichia coli strain HB-Coli0]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363