Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BCR17_RS03410 Genome accession   NZ_CP016598
Coordinates   699303..700670 (-) Length   455 a.a.
NCBI ID   WP_077281988.1    Uniprot ID   A0A5B8TEG9
Organism   Leuconostoc lactis strain WiKim40     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 694303..705670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCR17_RS03385 (BCR17_03385) - 694468..694974 (-) 507 WP_068851000.1 transposase -
  BCR17_RS03390 (BCR17_03390) adhP 695038..696093 (-) 1056 WP_029510101.1 alcohol dehydrogenase AdhP -
  BCR17_RS03395 (BCR17_03395) - 696256..697161 (-) 906 WP_068853027.1 NADP-dependent oxidoreductase -
  BCR17_RS03400 (BCR17_03400) gltX 697297..698793 (-) 1497 WP_068853031.1 glutamate--tRNA ligase -
  BCR17_RS03405 (BCR17_03405) - 698969..699292 (-) 324 WP_068851064.1 hypothetical protein -
  BCR17_RS03410 (BCR17_03410) radA 699303..700670 (-) 1368 WP_077281988.1 DNA repair protein RadA Machinery gene
  BCR17_RS03415 (BCR17_03415) - 700657..701538 (-) 882 WP_068851067.1 metallophosphoesterase family protein -
  BCR17_RS03420 (BCR17_03420) - 701773..702312 (-) 540 WP_048700325.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  BCR17_RS03425 (BCR17_03425) - 702538..702819 (+) 282 WP_010002509.1 GNAT family N-acetyltransferase -
  BCR17_RS03430 (BCR17_03430) rpiA 702816..703505 (+) 690 WP_029510095.1 ribose-5-phosphate isomerase RpiA -
  BCR17_RS03435 (BCR17_03435) - 703669..705093 (+) 1425 WP_068851070.1 aspartate ammonia-lyase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49227.40 Da        Isoelectric Point: 5.2659

>NTDB_id=189528 BCR17_RS03410 WP_077281988.1 699303..700670(-) (radA) [Leuconostoc lactis strain WiKim40]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIMPETNDRKSRVSLDGRTAKVEKINEVTSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IESLQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISIFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFVGEIGLTGE
IRSVADIESRLKEARKLGFKRAIVPKNNLNGVTLPEGIQVIGVATLREALQLALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=189528 BCR17_RS03410 WP_077281988.1 699303..700670(-) (radA) [Leuconostoc lactis strain WiKim40]
ATCGCAAAAGTTAAAACACAATTTATTTGCTCAAACTGTGGTTTCACGTCAGCGCGCTATCTGGGGCGCTGCTCAAACTG
TGGTGAATGGGGCACATTTGTCGAAGAAAAAATCATGCCCGAGACCAACGATCGGAAAAGTAGGGTGAGCCTAGACGGTC
GTACGGCTAAGGTTGAAAAGATTAATGAGGTGACATCAGAGGAAACACCGCGTGTTGCCACGAACCTTAAAGAACTGAAC
CGCGTGTTAGGTGGTGGCGTTGTGCCTGGGTCGATGGTGCTGATTGGTGGGGATCCAGGCATTGGGAAATCAACCCTCTT
GCTCCAAGTCTCTGGGCAATTGGCCCATGAAGGTCGTGTGTTATACGTGACTGGGGAAGAATCCGCCACCCAAGTGAAGC
TACGGGCAGATCGTTTGGGTGTTGGGAATGATGAATTTTACCTCTATCCAGAAACAGATATGACGGCGATTAAAAAGCAA
ATTGAATCGCTGCAACCAGATTTTGTCGTGATTGACTCAGTACAAACGATGCAGGAACCAGACGTGACGTCTGCCATTGG
CTCCGTGTCACAAATCCGCGAAGTCACGGCAGATTTATTGCAAATTGCCAAAACCAATAACATTTCTATTTTTATCGTTG
GTCACGTGACTAAAGATGGGGCGATTGCTGGACCAAAGATTTTGGAGCATATGGTCGATACGGTGCTCTATTTTGAAGGT
GATAGTAACTATAAGTACCGTATTTTACGTGCGGTGAAAAATCGTTTTGGTGCCACCAATGAACTGGGTATTTTTGAAAT
GCGTGACGGCGGCTTGATTGAAGTCGCCAACCCATCAGAAATCTTTTTGGAAGAACGGTTAGCTGGGGCAACTGGGTCGG
CCATTGTGGTCGCCTTGGAAGGCTCGCGGCCGATTTTGGTTGAATTACAGGCTTTAGTGACGCCCACCGTCTTTGGCAAT
GCGCAACGGACGGCATCCGGATTAGATCGTAACCGCGTGTCGTTAATTATGGCCGTTTTGGAAAAGCGCGCCAATCTCTT
GTTGCAAAATCAAGATGCTTATCTGAAAGCTGCCGGTGGGGTGAAACTGGATGAACCCGCAATTGATTTGGCGATTGCCG
TGGCACTGGCCAGTTCTTATCACGATAAAGAATCGCGGCCAAGTGACGTCTTTGTCGGTGAAATTGGGTTAACTGGGGAA
ATCCGGAGCGTCGCTGATATTGAAAGTCGGTTGAAGGAGGCGCGCAAGCTTGGCTTTAAACGGGCGATTGTACCAAAGAA
CAACTTGAATGGGGTCACACTTCCTGAAGGGATTCAAGTAATTGGCGTCGCCACGTTACGAGAAGCGTTGCAATTAGCCT
TAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8TEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.962

99.121

0.664
  radA Streptococcus pneumoniae D39

66.962

99.121

0.664
  radA Streptococcus pneumoniae R6

66.962

99.121

0.664
  radA Streptococcus pneumoniae TIGR4

66.962

99.121

0.664
  radA Streptococcus mitis NCTC 12261

66.741

99.121

0.662
  radA Streptococcus mitis SK321

66.741

99.121

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624

Multiple sequence alignment