Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BCM43_RS08875 Genome accession   NZ_CP016588
Coordinates   1687300..1687734 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain KNU-07     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1682300..1692734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCM43_RS08855 (BCM43_08855) - 1683214..1684377 (-) 1164 WP_000434578.1 aminotransferase A -
  BCM43_RS08860 (BCM43_08860) - 1684500..1684700 (-) 201 WP_000929277.1 hypothetical protein -
  BCM43_RS08865 (BCM43_08865) kinB 1685011..1686285 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  BCM43_RS08870 (BCM43_08870) - 1686324..1687073 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  BCM43_RS08875 (BCM43_08875) nucA/comI 1687300..1687734 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  BCM43_RS08880 (BCM43_08880) comJ 1687771..1688160 (+) 390 WP_000424030.1 competence protein ComJ -
  BCM43_RS08885 (BCM43_08885) metE 1688199..1690487 (-) 2289 WP_001007603.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BCM43_RS08890 (BCM43_08890) - 1690999..1691556 (-) 558 WP_000062063.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=189421 BCM43_RS08875 WP_000811494.1 1687300..1687734(+) (nucA/comI) [Bacillus thuringiensis strain KNU-07]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=189421 BCM43_RS08875 WP_000811494.1 1687300..1687734(+) (nucA/comI) [Bacillus thuringiensis strain KNU-07]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment