Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   BCM43_RS07425 Genome accession   NZ_CP016588
Coordinates   1428331..1429077 (+) Length   248 a.a.
NCBI ID   WP_000487013.1    Uniprot ID   A0A9W5QDK4
Organism   Bacillus thuringiensis strain KNU-07     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 1423331..1434077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCM43_RS07395 (BCM43_07395) - 1423620..1425764 (+) 2145 WP_000091514.1 HD family phosphohydrolase -
  BCM43_RS07400 (BCM43_07400) ybeY 1425761..1426231 (+) 471 WP_000054679.1 rRNA maturation RNase YbeY -
  BCM43_RS07405 (BCM43_07405) - 1426228..1426581 (+) 354 WP_000715469.1 diacylglycerol kinase family protein -
  BCM43_RS07410 (BCM43_07410) - 1426681..1427079 (+) 399 WP_001086282.1 cytidine deaminase -
  BCM43_RS07415 (BCM43_07415) era 1427072..1427977 (+) 906 WP_001080229.1 GTPase Era -
  BCM43_RS07420 (BCM43_07420) - 1428154..1428297 (+) 144 WP_000883924.1 YqzL family protein -
  BCM43_RS07425 (BCM43_07425) recO 1428331..1429077 (+) 747 WP_000487013.1 DNA repair protein RecO Machinery gene
  BCM43_RS07430 (BCM43_07430) - 1429185..1429817 (+) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  BCM43_RS07435 (BCM43_07435) - 1429847..1430659 (+) 813 WP_000368937.1 pyruvate, phosphate dikinase/phosphoenolpyruvate synthase regulator -
  BCM43_RS33160 - 1431295..1431438 (+) 144 WP_000479733.1 hypothetical protein -
  BCM43_RS07440 (BCM43_07440) dnaG 1431520..1433316 (+) 1797 WP_000528221.1 DNA primase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28609.20 Da        Isoelectric Point: 8.0488

>NTDB_id=189417 BCM43_RS07425 WP_000487013.1 1428331..1429077(+) (recO) [Bacillus thuringiensis strain KNU-07]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGQFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=189417 BCM43_RS07425 WP_000487013.1 1428331..1429077(+) (recO) [Bacillus thuringiensis strain KNU-07]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCAGTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGACATATTTTTAACTGCTTATGCATCATTTATTGTTGAATTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTTAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCGTATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment