Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BCM43_RS03190 Genome accession   NZ_CP016588
Coordinates   626686..628035 (+) Length   449 a.a.
NCBI ID   WP_000879428.1    Uniprot ID   W8YBC3
Organism   Bacillus thuringiensis strain KNU-07     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 621686..633035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCM43_RS03170 (BCM43_03170) - 622778..623413 (-) 636 WP_000926665.1 YigZ family protein -
  BCM43_RS03175 (BCM43_03175) - 623650..624492 (+) 843 WP_000684725.1 DegV family protein -
  BCM43_RS03180 (BCM43_03180) - 624665..624979 (+) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  BCM43_RS03185 (BCM43_03185) - 625128..626558 (+) 1431 WP_065702833.1 C40 family peptidase -
  BCM43_RS03190 (BCM43_03190) comFA 626686..628035 (+) 1350 WP_000879428.1 ATP-dependent helicase ComFA Machinery gene
  BCM43_RS03195 (BCM43_03195) - 628035..628739 (+) 705 WP_033683590.1 ComF family protein -
  BCM43_RS03200 (BCM43_03200) cspC 628866..629063 (+) 198 WP_001990088.1 cold shock protein CspC -
  BCM43_RS03205 (BCM43_03205) raiA 629386..629928 (+) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  BCM43_RS03210 (BCM43_03210) - 630333..630806 (+) 474 WP_065702835.1 hypothetical protein -
  BCM43_RS03215 (BCM43_03215) - 630924..631492 (+) 569 Protein_568 hypothetical protein -
  BCM43_RS03220 (BCM43_03220) - 631520..632308 (+) 789 WP_000120044.1 hypothetical protein -
  BCM43_RS03225 (BCM43_03225) - 632295..632990 (+) 696 WP_065702837.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51010.89 Da        Isoelectric Point: 9.9712

>NTDB_id=189407 BCM43_RS03190 WP_000879428.1 626686..628035(+) (comFA) [Bacillus thuringiensis strain KNU-07]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=189407 BCM43_RS03190 WP_000879428.1 626686..628035(+) (comFA) [Bacillus thuringiensis strain KNU-07]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCGCCAAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8YBC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372

Multiple sequence alignment