Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   E613_RS31000 Genome accession   NZ_CP020603
Coordinates   6369826..6372045 (+) Length   739 a.a.
NCBI ID   WP_003095847.1    Uniprot ID   Q9HUC9
Organism   Pseudomonas aeruginosa strain E6130952     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6364826..6377045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E613_RS30980 (E613_60410) - 6365801..6367069 (-) 1269 WP_003095842.1 malic enzyme -
  E613_RS30985 (E613_60420) - 6367172..6368611 (-) 1440 WP_003095844.1 M48 family metalloprotease -
  E613_RS30990 (E613_60430) - 6368608..6369375 (-) 768 WP_003095845.1 thermonuclease family protein -
  E613_RS30995 (E613_60440) rpmE 6369435..6369650 (-) 216 WP_003095846.1 50S ribosomal protein L31 -
  E613_RS31000 (E613_60450) priA 6369826..6372045 (+) 2220 WP_003095847.1 primosomal protein N' Machinery gene
  E613_RS31005 (E613_60460) argS 6372294..6374057 (+) 1764 WP_003095848.1 arginine--tRNA ligase -
  E613_RS31010 (E613_60470) - 6374092..6374787 (+) 696 WP_003095850.1 SPOR domain-containing protein -
  E613_RS31015 (E613_60480) hslV 6374906..6375439 (+) 534 WP_003095852.1 ATP-dependent protease subunit HslV -
  E613_RS31020 (E613_60490) hslU 6375468..6376811 (+) 1344 WP_003095854.1 ATP-dependent protease ATPase subunit HslU -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 82152.66 Da        Isoelectric Point: 8.7962

>NTDB_id=188701 E613_RS31000 WP_003095847.1 6369826..6372045(+) (priA) [Pseudomonas aeruginosa strain E6130952]
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLDAKPPMPAH
LLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHATAQSSLDDPRLARAPRQRQALAILKQHPHGVSHELL
NQLEINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRASHGGFHCFLLAGVTGSGKTEVYLQ
LIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRERLDAWLAARDGEADIVIGTRSALFTPLKRPGL
IIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGSATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKS
MPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRRGFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNC
PQCGKLDLRPLGAGTERAEERLRILFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAI
LDADGGLFSADFRASERMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHL
ALLRAEAHKPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=188701 E613_RS31000 WP_003095847.1 6369826..6372045(+) (priA) [Pseudomonas aeruginosa strain E6130952]
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGCGGCATTCC
GCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGCGTTCTGATAGAGGTGA
CGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGACGCCAAGCCGCCGATGCCGGCGCAC
CTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTCGGCGACACCCTGTCCTGGGCGCTGCCCAACCT
GTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGCTTCTGGCATGCCACCGCGCAATCCAGCCTCGACGATCCGC
GCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCCCTGGCGATTCTCAAGCAGCATCCGCACGGGGTTTCCCATGAGTTGCTG
AACCAACTGGAAATAAACAAGGACAGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAG
CACCCCGCCGCGCGAAGGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAGGCGG
TTCGCGCCAGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAGGCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGGCCCGCAGACCCTGGC
GCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAGCGCCTCGATGCCTGGC
TCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGCTCGGCACTGTTCACGCCGCTGAAGCGGCCGGGACTG
ATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGCCTGCGCTACCACGCCCGCGACCTGGCACTGGT
ACGCGCGCGCCTGGAGAATGTGCCGATCCTGCTCGGTTCGGCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCG
GCCGCTACGGCCTGCTGCGCCTGACCCAGCGCGCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGC
ATGCCGCTCGACGCCGGCCTTTCCCGTCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGT
ATTCCTCAATCGCCGCGGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACG
CGCGGATGACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAGTGCGGCAAGCTCGACCTGCGTCCGCTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATTCTCTTCCC
GAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTCTTCGCCACCATCAACA
GCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTCCCACGGGTCACCCTGGTGGCGATC
CTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAACGCATGGCCCAGCAGATCGTCCAGGTCGCCGG
CCGCGCAGGACGCGCCGAGGAGCCCGGGCGGGTACTGATCCAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCA
CCGAGGACGGCTACTTCGCCTTTGCCGAACAGGCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTC
GCCCTGCTGCGCGCCGAAGCGCACAAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATT
GCTCGAGCAGATGGGCGGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACC
GCGCGCAACTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA

Domains

Predicted by InterProScan.

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

DEAD/DEAH box helicase (DEAD)

(207-367)

Helicase conserved C-terminal domain (Helicase_C)

(522-592)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9HUC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.749

99.188

0.474