Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AWQ24_RS03600 Genome accession   NZ_CP016483
Coordinates   747562..749583 (+) Length   673 a.a.
NCBI ID   WP_065716035.1    Uniprot ID   -
Organism   Picosynechococcus sp. PCC 8807     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 742562..754583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ24_RS03580 (AWQ24_03580) - 742822..743076 (-) 255 WP_062432624.1 sulfurtransferase TusA family protein -
  AWQ24_RS03585 (AWQ24_03585) dnaJ 743073..744209 (-) 1137 WP_065710208.1 molecular chaperone DnaJ -
  AWQ24_RS03590 (AWQ24_03590) dnaK 744331..746286 (-) 1956 WP_065716033.1 molecular chaperone DnaK -
  AWQ24_RS03595 (AWQ24_03595) grpE 746403..747152 (-) 750 WP_065716034.1 nucleotide exchange factor GrpE -
  AWQ24_RS03600 (AWQ24_03600) pilB/pilB1 747562..749583 (+) 2022 WP_065716035.1 GspE/PulE family protein Machinery gene
  AWQ24_RS03605 (AWQ24_03605) pilT 749704..750810 (+) 1107 WP_012306326.1 type IV pilus twitching motility protein PilT Machinery gene
  AWQ24_RS03610 (AWQ24_03610) - 750876..752114 (+) 1239 WP_012306328.1 type II secretion system F family protein -
  AWQ24_RS03615 (AWQ24_03615) - 752123..752680 (+) 558 WP_012306329.1 hypothetical protein -
  AWQ24_RS03620 (AWQ24_03620) - 752677..753753 (-) 1077 WP_012306330.1 hypothetical protein -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75479.50 Da        Isoelectric Point: 5.2500

>NTDB_id=188533 AWQ24_RS03600 WP_065716035.1 747562..749583(+) (pilB/pilB1) [Picosynechococcus sp. PCC 8807]
MTHSSLPKRTRALALRNDFSPFGNKLIQQGVVDHQQMEKALVETRRSGRPLTAIVEELTGKALSPELVRQYKKHHLFELK
ILYGLNSIDPEVQKIDIDQMGELINSFVPVELCRRHRLLPIRRVQDEVIVAMVEPDNLAAQDDLNRLFRLQNLTLRRIVI
TEDDYRQVLDGYLEQQVQIQIQQQEQAAKAAEAAELRKAVDIEDLDLGVAGELDEQEEESADLMNENQASSAPVIKLVNK
VLLKALQEGASDIHIEPQENDLRIRFRKDGVLQYAFDPLPKRIVPAVTARFKIMADLDIAERRSPQDGRIRRMFKGRKVD
FRVNSLPSRYGEKIVLRILDNSSTQLGLDLLITNQNILGQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNEPGINI
STAEDPIEYTLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIA
RLDEMGVEPFMISGSLLGVLAQRLMRRVCGECRIPYQPTVEELARYGLSSNDRHPLTVYRANTLTPEQIQMAKEAGSLCP
KCGGGGYKGRVGVYEFMQNSEEIERLINTGATTDMIKEVAVQEGMLSILAYSLELVKEGHTTFEEVDRVTFTDSGLEAEL
KAKRKTGLVCDTCNAELQPDWLDCPYCMTPRFS

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=188533 AWQ24_RS03600 WP_065716035.1 747562..749583(+) (pilB/pilB1) [Picosynechococcus sp. PCC 8807]
ATGACCCACTCTTCCCTTCCCAAACGGACAAGAGCCCTTGCCCTCCGCAATGATTTCTCTCCTTTCGGCAACAAGCTCAT
TCAACAGGGAGTTGTAGACCATCAACAGATGGAAAAAGCCCTCGTTGAAACCCGGCGTAGTGGACGCCCCCTCACGGCCA
TTGTCGAAGAACTAACCGGGAAAGCCCTGTCCCCTGAGCTAGTCCGACAGTACAAAAAGCATCATCTCTTTGAACTGAAG
ATCCTCTACGGTCTCAATTCCATTGACCCAGAAGTTCAAAAAATTGACATCGATCAGATGGGCGAGTTGATCAATTCCTT
CGTGCCCGTAGAATTATGTCGCCGCCATCGTCTCCTCCCGATCCGGCGGGTTCAAGATGAAGTGATTGTGGCGATGGTAG
AGCCCGATAACCTAGCCGCCCAAGATGATTTAAACCGTCTTTTTCGCCTGCAAAATCTGACGTTACGGCGCATCGTGATC
ACAGAAGATGACTATCGTCAGGTTCTAGATGGCTATTTAGAACAGCAAGTCCAGATTCAAATCCAACAGCAGGAACAAGC
AGCCAAGGCCGCCGAAGCAGCGGAACTGAGAAAAGCAGTTGATATCGAAGATCTTGATCTCGGTGTAGCCGGGGAATTAG
ACGAGCAGGAAGAAGAAAGCGCCGACCTGATGAATGAAAATCAGGCTAGCTCGGCCCCGGTGATCAAACTCGTCAATAAA
GTGCTGCTCAAAGCCCTCCAGGAAGGGGCCTCAGATATTCACATCGAACCCCAAGAAAATGATCTGCGGATTCGTTTCCG
TAAAGATGGGGTTCTGCAATATGCTTTTGATCCGCTGCCCAAACGGATTGTGCCGGCGGTAACGGCCCGGTTTAAAATTA
TGGCGGACCTAGACATTGCGGAACGTCGTTCGCCTCAGGATGGTCGGATTCGGCGGATGTTTAAGGGGCGCAAGGTGGAC
TTTCGGGTCAATAGCCTACCCAGTCGCTACGGCGAAAAAATCGTTTTACGGATTCTCGATAATTCTTCAACCCAGTTGGG
CTTGGATTTGTTAATTACCAACCAAAATATTCTGGGGCAGGTGCGGGAAATGGCCCAGCGGCCATTTGGTCTAATTTTGG
TGACAGGGCCAACAGGCTCAGGAAAATCAACAACTTTGTATTCAATTCTGGCGGAGCGCAACGAGCCGGGCATCAACATC
AGTACCGCAGAAGACCCCATTGAATATACTTTGCCGGGCATTACCCAGGTGCAGGTCATCCGGGAGAAGGGAATGGATTT
TGCGTCAATTTTGCGGGCATTTTTGCGGCAGGATCCAGATGTCATTCTTGTGGGGGAAACCCGGGATAAGGAAACGGCAA
AAACAGCCATTGAAGCGGCTTTAACGGGGCACTTGGTACTAACGACTCTCCACACCAATGATGCTCCAGGGGCGATCGCC
CGTCTAGATGAGATGGGAGTTGAACCCTTTATGATTTCCGGGTCACTCCTGGGAGTTCTGGCCCAGCGCTTGATGCGACG
GGTTTGTGGGGAATGCCGCATCCCCTATCAGCCCACAGTGGAAGAGCTGGCACGCTACGGCCTTTCCAGTAATGATCGCC
ATCCTTTAACGGTGTACCGGGCCAATACCCTGACGCCAGAGCAAATCCAAATGGCCAAAGAAGCCGGCAGTCTCTGTCCT
AAATGTGGCGGCGGCGGCTACAAAGGCCGGGTGGGGGTCTATGAGTTTATGCAAAATAGTGAAGAAATTGAACGGTTGAT
TAATACCGGGGCCACCACGGACATGATTAAAGAAGTGGCAGTGCAAGAGGGCATGTTGAGTATCTTGGCCTACAGTTTGG
AGTTGGTCAAAGAAGGCCATACGACCTTTGAGGAGGTAGACCGTGTTACCTTTACTGATTCTGGCCTTGAGGCAGAACTA
AAGGCGAAACGAAAGACGGGCTTGGTTTGTGACACCTGTAATGCTGAGTTGCAGCCGGATTGGCTAGATTGTCCCTACTG
TATGACGCCCCGCTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(240-507)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.68

100

0.66

Multiple sequence alignment