Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   AWQ22_RS03560 Genome accession   NZ_CP016477
Coordinates   742303..744324 (+) Length   673 a.a.
NCBI ID   WP_065710209.1    Uniprot ID   A0AAN0YGZ0
Organism   Picosynechococcus sp. PCC 7117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 737303..749324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWQ22_RS03540 (AWQ22_03540) - 737564..737818 (-) 255 WP_065710207.1 sulfurtransferase TusA family protein -
  AWQ22_RS03545 (AWQ22_03545) dnaJ 737815..738951 (-) 1137 WP_065710208.1 molecular chaperone DnaJ -
  AWQ22_RS03550 (AWQ22_03550) dnaK 739073..741028 (-) 1956 WP_030005934.1 molecular chaperone DnaK -
  AWQ22_RS03555 (AWQ22_03555) grpE 741144..741893 (-) 750 WP_012306324.1 nucleotide exchange factor GrpE -
  AWQ22_RS03560 (AWQ22_03560) pilB/pilB1 742303..744324 (+) 2022 WP_065710209.1 GspE/PulE family protein Machinery gene
  AWQ22_RS03565 (AWQ22_03565) pilT 744444..745550 (+) 1107 WP_012306326.1 type IV pilus twitching motility protein PilT Machinery gene
  AWQ22_RS03570 (AWQ22_03570) - 745616..746854 (+) 1239 WP_012306328.1 type II secretion system F family protein -
  AWQ22_RS03575 (AWQ22_03575) - 746863..747420 (+) 558 WP_062432640.1 hypothetical protein -
  AWQ22_RS03580 (AWQ22_03580) - 747417..748493 (-) 1077 WP_065710210.1 glycosyl transferase -

Sequence

Protein


Download         Length: 673 a.a.        Molecular weight: 75374.45 Da        Isoelectric Point: 5.2500

>NTDB_id=188516 AWQ22_RS03560 WP_065710209.1 742303..744324(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7117]
MTHSSLPKRTRALALRNDFSPFGNKLIQQGVVDHQQMEKALVETRRSGRPLTAIVEELTGKALSPELVRQYKKHHLFELK
ILYGLNSIDPEVQKIDIDQMGELINSLVPVELCRRHRLLPIRRVQDEVIVAMVEPDNLAAQDDLNRLFRLQNLTLRRIVI
TEDDYRQVLDGYLEQQVQIQIQQQEQAAKAAEAAELKKAVDIEDLDLGVAGELDEQEEESADLMNENQASSAPVIKLVNK
VLLKALQEGASDIHIEPQENDLRIRFRKDGVLQYAFDPLPKRIVPAVTARFKIMADLDIAERRSPQDGRIRRMFKGRKVD
FRVNSLPSRYGEKIVLRILDNSSTQLGLDLLITNQDILGQVREMAQRPFGLILVTGPTGSGKSTTLYSILAERNEPGINI
STAEDPIEYTLPGITQVQVIREKGMDFASILRAFLRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIA
RLDEMGVEPFMISGSLLGVLAQRLMRRVCGECRIPYQPTVEELARYGLSSNDRHPLTVYRANTLTPEQIQMAKEAGSLCP
KCGGGGYKGRVGVYEFMQNSEEIERLINTGATTDMIKEVAVQEGMLSILAYSLELVKEGHTTFEEVDRVTFTDSGLEAEL
KAKRKTGLVCATCNAELQPDWLDCPYCMTPRFS

Nucleotide


Download         Length: 2022 bp        

>NTDB_id=188516 AWQ22_RS03560 WP_065710209.1 742303..744324(+) (pilB/pilB1) [Picosynechococcus sp. PCC 7117]
ATGACCCACTCTTCCCTTCCCAAACGGACAAGAGCCCTTGCCCTCCGCAATGATTTCTCTCCTTTCGGCAACAAGCTCAT
TCAACAGGGAGTTGTAGACCATCAACAGATGGAAAAAGCCCTCGTTGAAACCCGGCGTAGTGGACGCCCCCTCACGGCCA
TTGTCGAAGAACTAACCGGGAAAGCCCTGTCCCCTGAGCTAGTCCGACAGTACAAAAAGCATCATCTCTTTGAACTGAAG
ATCCTCTACGGTCTCAATTCCATTGACCCAGAAGTTCAAAAAATTGACATCGATCAGATGGGCGAGTTGATCAATTCCCT
CGTGCCCGTAGAATTATGTCGCCGCCACCGTCTCCTTCCGATCCGGCGGGTTCAAGATGAAGTGATTGTGGCGATGGTAG
AGCCCGATAACCTAGCCGCCCAAGATGATTTAAACCGTCTTTTTCGCCTGCAAAATCTGACGTTACGGCGCATCGTGATC
ACAGAAGACGACTATCGTCAGGTGCTAGATGGCTATTTAGAACAGCAAGTCCAGATTCAAATCCAACAGCAGGAACAAGC
AGCCAAGGCCGCCGAAGCAGCAGAACTGAAAAAAGCAGTTGATATCGAAGATCTTGATCTCGGTGTAGCCGGGGAATTAG
ACGAGCAGGAAGAAGAAAGCGCCGACCTGATGAATGAAAATCAGGCTAGCTCTGCTCCGGTGATCAAACTCGTCAATAAA
GTGCTGCTCAAAGCCCTCCAGGAAGGGGCCTCAGATATTCACATCGAACCCCAAGAAAATGATCTGCGGATTCGTTTCCG
TAAAGATGGGGTTCTGCAATATGCTTTTGATCCGCTGCCCAAACGGATTGTGCCGGCGGTGACGGCCCGGTTTAAAATTA
TGGCGGACCTAGACATTGCGGAACGTCGTTCGCCTCAGGATGGTCGGATTCGGCGGATGTTTAAGGGGCGCAAGGTGGAC
TTTCGGGTCAATAGCCTACCCAGTCGCTACGGCGAAAAAATTGTTTTACGGATTCTCGATAATTCTTCAACCCAGTTGGG
CTTGGATTTGTTAATTACCAACCAAGATATTCTGGGGCAGGTGCGGGAAATGGCCCAGCGGCCATTTGGTCTAATTTTGG
TGACGGGGCCAACAGGCTCAGGAAAATCAACAACTTTGTATTCAATTCTGGCGGAGCGCAACGAGCCGGGCATCAACATC
AGTACCGCAGAAGACCCCATTGAATATACTTTGCCGGGCATTACCCAGGTGCAGGTCATCCGGGAGAAGGGGATGGATTT
TGCGTCAATTTTGCGGGCATTTTTGCGGCAGGATCCAGATGTCATTCTTGTGGGGGAAACCCGGGATAAGGAAACGGCAA
AAACAGCCATTGAGGCGGCTTTAACGGGGCACTTGGTACTAACGACTCTCCACACCAATGATGCTCCAGGGGCGATCGCC
CGTCTAGATGAGATGGGAGTTGAACCCTTTATGATTTCCGGGTCACTCCTGGGAGTTCTGGCCCAGCGCTTGATGCGACG
GGTTTGTGGGGAATGCCGCATCCCCTATCAGCCCACAGTGGAAGAGCTGGCACGCTACGGCCTTTCCAGTAATGACCGCC
ATCCTTTAACGGTGTACCGGGCCAATACCCTGACGCCAGAGCAAATCCAAATGGCCAAAGAAGCCGGCAGTCTCTGTCCT
AAATGTGGCGGCGGCGGCTACAAAGGCCGGGTGGGGGTCTATGAGTTTATGCAAAATAGTGAAGAAATTGAACGGTTGAT
TAATACCGGGGCCACCACGGACATGATTAAAGAAGTGGCAGTGCAAGAGGGCATGTTGAGTATCTTGGCCTACAGTTTGG
AGTTGGTCAAAGAAGGCCATACGACCTTTGAGGAGGTAGACCGTGTTACCTTTACGGATTCTGGCCTTGAGGCAGAACTA
AAGGCGAAACGAAAAACGGGCCTGGTTTGTGCCACCTGTAATGCTGAGTTGCAGCCGGATTGGCTAGATTGTCCCTACTG
TATGACGCCCCGCTTTAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(240-507)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.533

100

0.658

Multiple sequence alignment