Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A4W81_RS01125 Genome accession   NZ_CP016465
Coordinates   226301..227665 (+) Length   454 a.a.
NCBI ID   WP_011375373.1    Uniprot ID   A0A094XXJ4
Organism   Latilactobacillus sakei strain TMW 1.3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 221301..232665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W81_RS01105 (A4W81_01095) - 221462..222808 (-) 1347 WP_076632142.1 C1 family peptidase -
  A4W81_RS01110 (A4W81_01100) - 223013..224335 (-) 1323 WP_076632143.1 C1 family peptidase -
  A4W81_RS01115 (A4W81_01105) rpiA 224810..225493 (-) 684 WP_011375375.1 ribose-5-phosphate isomerase RpiA -
  A4W81_RS01120 (A4W81_01110) - 225689..226228 (+) 540 WP_011375374.1 dUTP diphosphatase -
  A4W81_RS01125 (A4W81_01115) radA 226301..227665 (+) 1365 WP_011375373.1 DNA repair protein RadA Machinery gene
  A4W81_RS01130 (A4W81_01120) - 227767..228807 (+) 1041 WP_350028516.1 PIN/TRAM domain-containing protein -
  A4W81_RS01135 (A4W81_01125) cysS 229238..230644 (+) 1407 WP_056947506.1 cysteine--tRNA ligase -
  A4W81_RS01140 (A4W81_01130) - 230641..231048 (+) 408 WP_004265086.1 Mini-ribonuclease 3 -
  A4W81_RS01145 (A4W81_01135) rlmB 231035..231922 (+) 888 WP_082267809.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  A4W81_RS01150 (A4W81_01140) - 231926..232458 (+) 533 Protein_207 NYN domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49598.97 Da        Isoelectric Point: 7.3488

>NTDB_id=188400 A4W81_RS01125 WP_011375373.1 226301..227665(+) (radA) [Latilactobacillus sakei strain TMW 1.3]
MAKVKTQFVCQNCGYSSPRFLGRCPNCGAWNQMVEEREQPAAAAKSNFTISGRATEPEKISTVNIQKEPRVKTELNELNR
VLGGGVVPGSLILIGGDPGIGKSTLLLQVSGQLEKVGKILYVSGEESASQIKMRANRLGVNGDQLYLYPETDMGNIRHQI
ETLKPEYVVIDSIQTMSEPEVTSAVGSVSQVRQVTAELMRIAKTNQITIFVVGHVTKEGAIAGPKILEHMVDTVLYFEGD
THHTYRILRSVKNRFGSTNEIGIFEMREAGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVELQTLITPTLFGNA
KRTSSGLDHNRVSLIMAVLEKRASLMLSNQDAYLKATGGVKLDEPAIDLAMAVSIASSYRDKEIPPTDCFVGEIGLTGEI
RRVNRIEQRVGEAAKLGFKRIYVPKNNLQGWDPPTDIQVVGVTTIAETLKKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=188400 A4W81_RS01125 WP_011375373.1 226301..227665(+) (radA) [Latilactobacillus sakei strain TMW 1.3]
GTGGCTAAAGTTAAAACGCAGTTTGTCTGCCAGAATTGTGGCTATAGTTCCCCCAGATTCTTGGGGCGCTGTCCGAATTG
TGGTGCTTGGAACCAAATGGTTGAAGAACGTGAACAACCAGCCGCAGCGGCAAAATCAAATTTCACAATTTCAGGGCGCG
CCACTGAACCTGAAAAAATTAGTACCGTTAATATCCAAAAAGAACCCCGTGTTAAAACGGAATTAAACGAATTGAATCGT
GTTTTAGGTGGCGGCGTTGTGCCGGGGTCACTGATTTTAATCGGTGGGGATCCTGGGATTGGGAAATCAACCTTGTTACT
ACAAGTTTCAGGGCAACTTGAAAAAGTGGGTAAGATCCTATACGTTTCAGGTGAAGAAAGTGCCTCGCAAATTAAGATGC
GGGCGAATCGATTAGGAGTTAATGGCGATCAATTGTATCTGTATCCCGAAACGGATATGGGCAATATTCGTCACCAAATT
GAAACGCTCAAACCGGAATACGTCGTGATTGATTCGATTCAAACGATGAGTGAACCAGAAGTGACTTCCGCAGTCGGGAG
TGTTTCGCAAGTTCGCCAAGTAACCGCAGAATTGATGCGGATTGCTAAAACGAATCAAATTACCATCTTCGTGGTAGGAC
ATGTGACGAAGGAAGGGGCGATTGCCGGCCCTAAGATTTTGGAACATATGGTAGATACCGTGTTGTATTTTGAAGGTGAC
ACCCATCACACTTACCGGATTTTACGATCTGTTAAAAACCGGTTTGGTTCAACTAATGAAATCGGGATTTTCGAAATGCG
GGAAGCGGGCTTGCAAGAAGTCGCTAATCCATCCGAAATTTTCCTTGAAGAACGACTGGCCGGTGCGACTGGTTCAGCAG
TTGTGGTTTCAATGGAAGGGACAAGACCAATCTTGGTTGAGTTACAAACTTTGATTACCCCAACCTTGTTCGGGAATGCG
AAACGGACCTCTTCAGGGTTAGATCATAATCGGGTGTCATTGATTATGGCGGTTTTAGAAAAGCGGGCGAGTTTGATGCT
TTCAAACCAAGATGCTTATCTTAAAGCAACCGGTGGCGTTAAGTTAGATGAACCAGCGATTGATTTGGCGATGGCCGTTT
CAATTGCTTCCAGTTATCGCGATAAAGAAATTCCACCAACCGATTGTTTTGTCGGCGAAATTGGCTTGACGGGTGAAATC
CGGCGGGTCAACCGGATCGAACAACGCGTTGGCGAAGCGGCGAAGTTAGGTTTTAAACGGATTTATGTTCCTAAAAATAA
TTTGCAAGGTTGGGATCCACCAACTGATATTCAAGTCGTTGGGGTCACCACTATTGCAGAAACTTTAAAAAAAGTATTCA
ACTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

AAA domain (AAA_25)

(70-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A094XXJ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

69.316

99.78

0.692
  radA Streptococcus mitis SK321

69.316

99.78

0.692
  radA Streptococcus pneumoniae Rx1

69.095

99.78

0.689
  radA Streptococcus pneumoniae D39

69.095

99.78

0.689
  radA Streptococcus pneumoniae R6

69.095

99.78

0.689
  radA Streptococcus pneumoniae TIGR4

69.095

99.78

0.689
  radA Bacillus subtilis subsp. subtilis str. 168

65.121

99.78

0.65

Multiple sequence alignment