Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BBJ41_RS14810 Genome accession   NZ_CP016442
Coordinates   3177178..3178683 (-) Length   501 a.a.
NCBI ID   WP_069747014.1    Uniprot ID   -
Organism   Burkholderia stabilis strain ATCC BAA-67     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3172178..3183683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBJ41_RS14785 (BBJ41_14785) - 3173206..3173841 (-) 636 WP_069747011.1 LON peptidase substrate-binding domain-containing protein -
  BBJ41_RS14790 (BBJ41_14790) rapZ 3173870..3174778 (-) 909 WP_069747012.1 RNase adapter RapZ -
  BBJ41_RS14795 (BBJ41_14795) hprK 3174854..3175822 (-) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  BBJ41_RS14800 (BBJ41_14800) - 3175952..3176491 (-) 540 WP_069747013.1 PTS sugar transporter subunit IIA -
  BBJ41_RS14805 (BBJ41_14805) hpf 3176660..3177019 (-) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BBJ41_RS14810 (BBJ41_14810) rpoN/rpoN1 3177178..3178683 (-) 1506 WP_069747014.1 RNA polymerase factor sigma-54 Machinery gene
  BBJ41_RS14815 (BBJ41_14815) lptB 3178844..3179620 (-) 777 WP_069747015.1 LPS export ABC transporter ATP-binding protein -
  BBJ41_RS14820 (BBJ41_14820) lptA 3179617..3180282 (-) 666 WP_069747016.1 lipopolysaccharide transport periplasmic protein LptA -
  BBJ41_RS14825 (BBJ41_14825) lptC 3180303..3180917 (-) 615 WP_069747017.1 LPS export ABC transporter periplasmic protein LptC -
  BBJ41_RS14830 (BBJ41_14830) - 3180921..3181457 (-) 537 WP_069747018.1 KdsC family phosphatase -
  BBJ41_RS14835 (BBJ41_14835) kdsD 3181457..3182440 (-) 984 WP_069747019.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54814.88 Da        Isoelectric Point: 4.8740

>NTDB_id=188305 BBJ41_RS14810 WP_069747014.1 3177178..3178683(-) (rpoN/rpoN1) [Burkholderia stabilis strain ATCC BAA-67]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVASDGSLIAQTPPAQNTEPSA
ANGSSSSGDRAERDEPAGADEYDGYSSNDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=188305 BBJ41_RS14810 WP_069747014.1 3177178..3178683(-) (rpoN/rpoN1) [Burkholderia stabilis strain ATCC BAA-67]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGTCGGACGGATCGCTGATCGCGCAGACGCCTCCGGCACAGAACACCGAACCGTCCGCG
GCAAACGGCAGCAGCTCGTCGGGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACTCGTC
CAACGACGGCAACGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCAGGAAGCCGCAACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAGGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGATGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCTG
CCGGGGTCGGCGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTGGAACTGCTCGCCGCACGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAAG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCCGCCGGTTCGCTGAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAAAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAGAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGATAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCCGTCAATCTGCGAAAGTCTCTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.577

100

0.693

Multiple sequence alignment