Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BBN53_RS00470 Genome accession   NZ_CP016440
Coordinates   111590..113026 (+) Length   478 a.a.
NCBI ID   WP_082149548.1    Uniprot ID   -
Organism   Bordetella pseudohinzii strain HI4681     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 106590..118026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBN53_RS00455 (BBN53_00455) - 107451..108740 (+) 1290 WP_043211125.1 amidase -
  BBN53_RS00460 (BBN53_00460) leuA 108836..110545 (-) 1710 WP_043210604.1 2-isopropylmalate synthase -
  BBN53_RS00465 (BBN53_00465) - 110920..111591 (-) 672 WP_043210602.1 hypothetical protein -
  BBN53_RS00470 (BBN53_00470) rpoN/rpoN1 111590..113026 (+) 1437 WP_082149548.1 RNA polymerase factor sigma-54 Machinery gene
  BBN53_RS00475 (BBN53_00475) - 113122..113418 (+) 297 WP_109842466.1 (2Fe-2S)-binding protein -
  BBN53_RS00480 (BBN53_00480) - 113481..114350 (-) 870 WP_048025970.1 LysR family transcriptional regulator -
  BBN53_RS00485 (BBN53_00485) - 114457..115368 (+) 912 WP_043210596.1 DMT family transporter -
  BBN53_RS00490 (BBN53_00490) bfr 115680..116156 (+) 477 WP_043210594.1 bacterioferritin -
  BBN53_RS00495 (BBN53_00495) - 116220..116909 (-) 690 WP_180961708.1 hypothetical protein -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 52733.99 Da        Isoelectric Point: 4.9251

>NTDB_id=188281 BBN53_RS00470 WP_082149548.1 111590..113026(+) (rpoN/rpoN1) [Bordetella pseudohinzii strain HI4681]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAEISQALAENPLLERDETPADQVESDPQREASVEHDDSA
PDTELRFDEMPGSGGVYPDDDSDAPARDDTLREHLMQQLNLTRAAPRDAALVTLLIDELDDNGYLGSPLSEILQWLPPEL
EVDEDELRAALNLLQSFDPPGIGARDMAECLLLQLRQPDIQRLPEAAQAGVLACAREICAQHLALLATGNPARLREVLRV
DEATLRAARALIVRLEPRPGRAWTAPAADYAVPDVLVRKTRQGWQAVLNSAVMPRLQINGLYAQMLSNQREAAYAGLNAQ
LQQARWMIRNVEQRFDTILRVAQAIVAHQAEFFSRGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLELKRF
FGTGVATDSGDSASATAVQAHIRQMVAEENRARPLSDSQIMERLASQGIVIARRTVAKYREALRIAPAAVRKAQAAAR

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=188281 BBN53_RS00470 WP_082149548.1 111590..113026(+) (rpoN/rpoN1) [Bordetella pseudohinzii strain HI4681]
ATGCAAAATATTGTACCTATGACTCGTCCCGCCTTAGAACTTCGGCCTGGTCAGCATTTGACCCTGACCCCGCAGCTGCA
ACAGTCCATCCGGCTGCTGCAGCTTTCTTCGCTGGAGCTGGAGGCGGAAATCTCCCAGGCCCTGGCGGAAAATCCGTTGT
TGGAACGCGACGAAACTCCGGCCGACCAGGTCGAGAGCGACCCCCAGCGCGAGGCCAGCGTCGAACACGACGACAGCGCC
CCGGACACGGAATTGCGTTTCGACGAGATGCCGGGCTCGGGCGGCGTCTATCCGGACGACGACAGCGATGCGCCGGCGCG
CGACGACACCCTGCGCGAGCATCTGATGCAGCAGCTGAACCTGACGCGCGCGGCCCCGCGCGATGCCGCCCTGGTCACCC
TGCTCATCGACGAACTGGATGACAACGGCTACCTGGGCTCGCCGCTGAGCGAAATCCTGCAATGGCTGCCCCCCGAACTG
GAGGTCGATGAGGATGAGCTGCGCGCGGCCCTGAATCTGCTGCAGTCCTTCGATCCGCCCGGCATCGGCGCGCGCGACAT
GGCCGAGTGCCTGCTCCTGCAGCTGCGCCAGCCCGATATCCAGCGCCTGCCCGAAGCCGCGCAAGCCGGGGTGCTGGCCT
GTGCGCGGGAGATCTGCGCGCAGCACCTGGCGCTCCTGGCCACCGGCAATCCGGCGCGCCTGCGCGAGGTCCTGCGCGTG
GACGAGGCCACGCTGCGCGCGGCGCGCGCGCTCATCGTGCGGCTGGAGCCCCGGCCCGGCCGGGCCTGGACCGCGCCGGC
CGCGGACTACGCCGTGCCCGACGTGCTGGTGCGCAAGACGCGCCAGGGCTGGCAGGCCGTGCTCAACAGCGCGGTGATGC
CCCGCCTGCAGATCAACGGCCTGTACGCCCAGATGCTGTCCAACCAGCGCGAAGCGGCCTATGCGGGCCTGAACGCCCAG
CTCCAGCAGGCGCGCTGGATGATACGCAATGTCGAGCAGCGCTTTGACACCATCTTGCGCGTGGCCCAGGCCATCGTCGC
GCACCAGGCGGAGTTCTTCAGCCGCGGGCCCGCCGCCATGCGACCCCTCATCCTCAAGGACATCGCGGGCGAGCTAGGCC
TGCACGAATCCACGATTTCCCGCGCTACGACCCAGAAGTACATGCTCACTCCGTTTGGCACGCTGGAGCTCAAGCGCTTT
TTCGGCACGGGCGTGGCGACCGACAGCGGCGACTCCGCCTCGGCCACGGCGGTGCAGGCCCATATCCGGCAGATGGTGGC
CGAAGAAAACAGGGCCAGACCCCTGTCGGATAGCCAGATCATGGAGCGCCTGGCCAGCCAGGGCATCGTGATCGCCCGCC
GCACCGTGGCCAAATACCGTGAGGCCCTGCGTATCGCCCCGGCCGCGGTGCGCAAGGCCCAGGCCGCGGCGCGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-302)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-472)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.802

100

0.527

Multiple sequence alignment