Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BBB44_RS23335 Genome accession   NZ_CP016431
Coordinates   4969267..4970706 (-) Length   479 a.a.
NCBI ID   WP_050458203.1    Uniprot ID   -
Organism   Bordetella bronchiseptica strain I328     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4964267..4975706
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBB44_RS23310 (BBB44_23310) - 4965346..4966029 (+) 684 WP_080699941.1 hypothetical protein -
  BBB44_RS23315 (BBB44_23315) bfr 4966114..4966590 (-) 477 WP_003815898.1 bacterioferritin -
  BBB44_RS23320 (BBB44_23320) - 4966929..4967858 (-) 930 WP_033467029.1 DMT family transporter -
  BBB44_RS23325 (BBB44_23325) - 4967966..4968844 (+) 879 WP_033466910.1 LysR substrate-binding domain-containing protein -
  BBB44_RS23330 (BBB44_23330) - 4968872..4969174 (-) 303 WP_003815905.1 bacterioferritin-associated ferredoxin -
  BBB44_RS23335 (BBB44_23335) rpoN/rpoN1 4969267..4970706 (-) 1440 WP_050458203.1 RNA polymerase factor sigma-54 Machinery gene
  BBB44_RS23340 (BBB44_23340) - 4970705..4971352 (+) 648 WP_033466912.1 hypothetical protein -
  BBB44_RS23345 (BBB44_23345) leuA 4971779..4973485 (+) 1707 WP_033466914.1 2-isopropylmalate synthase -
  BBB44_RS23350 (BBB44_23350) - 4973672..4974955 (-) 1284 WP_033466916.1 amidase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52395.60 Da        Isoelectric Point: 4.8206

>NTDB_id=188203 BBB44_RS23335 WP_050458203.1 4969267..4970706(-) (rpoN/rpoN1) [Bordetella bronchiseptica strain I328]
MQNIVPMTRPALELRPGQHLTLTPQLQQSIRLLQLSSLELEAELSQALAENPLLERDEDPAREEQADPEREASVEADEAP
DTELRLDEMPGSGGVHPDDDSDLPQAARPDTLREHLLGQLALTRAAPRDAALCGLLIDELDENGYLDSPLEEILTWLPAE
AEADLDELRAVLTLLQSFDPVGIGARDMAECLQLQLRQPDLARLPEAADAEVLACARRICAQHLPLLATGNVARVREAVQ
CDEAVVRAAHALILRLEPRPGRAWTVPAADYAIPDVIVRRTRQGWQAVLNSAVVPRLHVNAVYAQVLGGARQGGNAGLQA
QLQQARWMIRNVEQRFDTILRVSQAIVAHQQAFFSQGPAAMRPLILKDIAGELDLHESTISRATTQKYMLTPFGTLELKR
FFGAGVSTDGGDATSATAVQAVIRQLVADEDRAKPLSDSQIMQRLADQGIVIARRTVAKYREALRIAPATLRKAHAAGR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=188203 BBB44_RS23335 WP_050458203.1 4969267..4970706(-) (rpoN/rpoN1) [Bordetella bronchiseptica strain I328]
ATGCAAAATATTGTACCTATGACTCGTCCCGCGCTAGAACTTCGTCCTGGTCAACACCTGACATTGACCCCTCAACTGCA
ACAGTCGATCCGGCTGTTGCAGCTGTCGTCGCTGGAGCTGGAGGCCGAACTGTCGCAGGCGCTGGCCGAGAATCCGTTGC
TGGAACGCGACGAAGACCCGGCGCGCGAGGAGCAGGCCGACCCCGAGCGCGAGGCCTCGGTCGAGGCCGACGAGGCGCCG
GACACCGAATTGCGCCTGGACGAGATGCCCGGTTCGGGCGGCGTCCATCCCGACGACGACAGCGACCTGCCGCAGGCGGC
GCGCCCGGATACCTTGCGCGAGCACCTGCTGGGGCAGTTGGCGCTGACGCGCGCCGCGCCGCGCGACGCGGCGCTGTGCG
GCTTGCTGATCGACGAACTGGACGAGAACGGCTACCTGGATTCGCCCCTGGAAGAAATCCTGACCTGGCTGCCGGCCGAG
GCGGAGGCCGACCTGGACGAACTGCGCGCAGTGCTGACATTGCTGCAATCGTTCGACCCGGTGGGCATCGGCGCGCGCGA
CATGGCCGAATGCCTGCAGCTGCAGCTGCGCCAGCCCGATCTGGCGCGTCTGCCCGAGGCGGCCGACGCCGAGGTGCTGG
CCTGTGCGCGGCGCATCTGCGCGCAGCACCTGCCCTTGCTGGCCACCGGCAACGTGGCCCGCGTGCGCGAGGCCGTGCAA
TGCGACGAGGCGGTCGTGCGCGCTGCGCATGCGCTGATCCTGCGCCTGGAGCCGCGGCCGGGCCGCGCCTGGACGGTGCC
GGCGGCCGATTACGCCATTCCCGACGTGATCGTGCGCCGTACGCGCCAGGGCTGGCAGGCGGTGCTCAACAGCGCCGTCG
TGCCGCGGCTGCACGTCAATGCCGTCTATGCGCAGGTGCTGGGGGGCGCGCGCCAGGGCGGCAACGCCGGCCTGCAGGCG
CAGTTGCAACAGGCGCGCTGGATGATACGCAACGTGGAGCAGCGCTTCGACACCATCCTGCGCGTCTCGCAGGCGATCGT
GGCGCATCAGCAGGCGTTCTTCAGCCAGGGGCCGGCGGCCATGCGGCCGCTGATCCTCAAGGACATCGCCGGCGAACTGG
ATCTGCACGAATCGACCATTTCGCGGGCCACGACGCAGAAGTACATGCTCACACCGTTTGGCACGCTGGAGCTAAAGCGC
TTCTTCGGCGCGGGCGTGTCCACCGACGGCGGCGACGCCACCTCGGCCACCGCGGTGCAGGCGGTGATCCGCCAGCTGGT
GGCCGACGAGGATCGCGCCAAGCCCCTGTCGGACAGCCAGATCATGCAGCGCCTGGCCGACCAGGGCATCGTCATTGCCC
GCCGCACGGTGGCCAAGTACCGCGAGGCGCTGCGGATTGCGCCAGCGACGCTACGCAAGGCCCATGCGGCGGGTCGTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-473)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.419

100

0.511

Multiple sequence alignment