Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BBJ33_RS08940 Genome accession   NZ_CP016395
Coordinates   1849490..1849924 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus velezensis strain M75     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1844490..1854924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBJ33_RS08935 (BBJ33_08935) - 1847990..1849201 (-) 1212 WP_069473684.1 cytochrome P450 -
  BBJ33_RS08940 (BBJ33_08940) nucA/comI 1849490..1849924 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  BBJ33_RS08945 (BBJ33_08945) - 1849984..1850751 (+) 768 WP_069473422.1 YoaK family protein -
  BBJ33_RS08950 (BBJ33_08950) - 1850772..1851134 (-) 363 WP_003154082.1 hypothetical protein -
  BBJ33_RS08955 (BBJ33_08955) - 1851326..1852654 (-) 1329 WP_069473423.1 S8 family peptidase -
  BBJ33_RS08960 (BBJ33_08960) - 1852834..1853067 (+) 234 WP_046341482.1 hypothetical protein -
  BBJ33_RS08965 (BBJ33_08965) - 1853323..1854030 (+) 708 WP_046341256.1 poly-gamma-glutamate hydrolase family protein -
  BBJ33_RS08970 (BBJ33_08970) - 1854090..1854542 (+) 453 WP_015388205.1 OsmC family protein -
  BBJ33_RS08975 (BBJ33_08975) - 1854556..1854909 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=187908 BBJ33_RS08940 WP_003154085.1 1849490..1849924(+) (nucA/comI) [Bacillus velezensis strain M75]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=187908 BBJ33_RS08940 WP_003154085.1 1849490..1849924(+) (nucA/comI) [Bacillus velezensis strain M75]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment