Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   TK34_RS01910 Genome accession   NZ_CP016380
Coordinates   424089..425528 (+) Length   479 a.a.
NCBI ID   WP_065475309.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AHNIH1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419089..430528
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK34_RS01880 (TK34_01860) mlaF 419665..420465 (-) 801 WP_011707618.1 phospholipid ABC transporter ATP-binding protein MlaF -
  TK34_RS01885 (TK34_01865) - 420674..421669 (+) 996 WP_017409369.1 KpsF/GutQ family sugar-phosphate isomerase -
  TK34_RS01890 (TK34_01870) kdsC 421669..422223 (+) 555 WP_017409370.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  TK34_RS01895 (TK34_01875) lptC 422220..422780 (+) 561 WP_011707615.1 LPS export ABC transporter periplasmic protein LptC -
  TK34_RS01900 (TK34_01880) lptA 422767..423294 (+) 528 WP_016352198.1 lipopolysaccharide transport periplasmic protein LptA -
  TK34_RS01905 (TK34_01885) lptB 423298..424023 (+) 726 WP_011707613.1 LPS export ABC transporter ATP-binding protein -
  TK34_RS01910 (TK34_01890) rpoN/rpoN1 424089..425528 (+) 1440 WP_065475309.1 RNA polymerase factor sigma-54 Machinery gene
  TK34_RS01915 (TK34_01895) hpf 425550..425837 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  TK34_RS01920 (TK34_01900) ptsN 425840..426286 (+) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  TK34_RS01925 (TK34_01905) rapZ 426329..427195 (+) 867 WP_011707611.1 RNase adapter RapZ -
  TK34_RS01930 (TK34_01910) npr 427216..427488 (+) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  TK34_RS01935 (TK34_01915) - 427504..429408 (-) 1905 WP_065475311.1 BglG family transcription antiterminator -
  TK34_RS01940 (TK34_01920) dagF 429513..430253 (-) 741 WP_065475313.1 2-dehydro-3-deoxy-phosphogluconate aldolase -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53824.71 Da        Isoelectric Point: 4.3467

>NTDB_id=187626 TK34_RS01910 WP_065475309.1 424089..425528(+) (rpoN/rpoN1) [Aeromonas hydrophila strain AHNIH1]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSPEEPVDASQLDSSDAMSQDNMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQTAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAKEVILEHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=187626 TK34_RS01910 WP_065475309.1 424089..425528(+) (rpoN/rpoN1) [Aeromonas hydrophila strain AHNIH1]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAATGAGG
CGCAGGAGACCAGCCCGGAAGAGCCGGTCGATGCCAGCCAACTTGACTCCAGCGATGCCATGTCTCAGGACAACATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGAGGTCTACTCGGCCGGTACGGCCCAGAGCGCGGGCCCGATCCACGACGG
TGAAGACAGCATCTATCAGGGCGAAACCACCGAAAACCTGCAGGATTATCTGTTGTGGCAGATGCGCCTGACGCCCTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATCGACGCCATCGACGAATCCGGTTACCTGACGGTGTCGCTGGAAGAT
ATCCAGACTGCGGTAAGCAGCGAGGATGTCGAGGTGGAGCTCGATGAAGTGGAGGCGGTGCTCAAGCGGGTGCAGCACTT
TGATCCGGTCGGCATCGCCGCTCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTGGGCCAATACTCCCCGGACTTGCCCT
GGCTGAACGAGGCCAAGGAGGTGATCCTTGAGCACATGGACTTGCTCGGCAATCGCGACTACCGCACCCTGGCGCGCAAG
ACCCGACTCAAGGAGAGCGACCTCAAGGAGGTGCTGGACTTGATCCAGCAGCTGGAGCCAAGACCCGGCAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAATCCGG
ACTCGGCCCCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCTCGCACCAGCCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAGAGCCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGTATCGTCGAGCAGCAGCAGGGCTTTTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTCACGACCCAGAAGTACATGCATACACCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.455

100

0.48

Multiple sequence alignment