Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   AWB10_RS00485 Genome accession   NZ_CP020509
Coordinates   90851..91519 (+) Length   222 a.a.
NCBI ID   WP_001352775.1    Uniprot ID   -
Organism   Escherichia coli strain 165     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 85851..96519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWB10_RS00450 (AWB10_00450) yicC 85870..86733 (-) 864 WP_000621336.1 YicC/YloC family endoribonuclease -
  AWB10_RS00460 (AWB10_00460) rph 86860..87576 (+) 717 WP_001247093.1 ribonuclease PH -
  AWB10_RS00465 (AWB10_00465) pyrE 87642..88283 (+) 642 WP_000806162.1 orotate phosphoribosyltransferase -
  AWB10_RS00470 (AWB10_00470) slmA 88320..88916 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  AWB10_RS00475 (AWB10_00475) dut 89023..89481 (-) 459 WP_000976070.1 dUTP diphosphatase -
  AWB10_RS00480 (AWB10_00480) coaBC 89459..90679 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  AWB10_RS00485 (AWB10_00485) radC 90851..91519 (+) 669 WP_001352775.1 DNA repair protein RadC Machinery gene
  AWB10_RS00490 (AWB10_00490) rpmB 91736..91972 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  AWB10_RS00495 (AWB10_00495) rpmG 91993..92160 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  AWB10_RS00500 (AWB10_00500) mutM 92258..93067 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  AWB10_RS00505 (AWB10_00505) coaD 93106..93585 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  AWB10_RS00510 (AWB10_00510) waaA 93593..94870 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  AWB10_RS00515 (AWB10_00515) rfaQ 95283..96341 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25292.38 Da        Isoelectric Point: 7.9748

>NTDB_id=187063 AWB10_RS00485 WP_001352775.1 90851..91519(+) (radC) [Escherichia coli strain 165]
MKNNSQLMMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=187063 AWB10_RS00485 WP_001352775.1 90851..91519(+) (radC) [Escherichia coli strain 165]
GTGAAAAACAATTCACAGCTGATGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(7-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment