Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A9176_RS01900 Genome accession   NZ_CP016329
Coordinates   379170..380537 (-) Length   455 a.a.
NCBI ID   WP_077281988.1    Uniprot ID   A0A5B8TEG9
Organism   Leuconostoc garlicum strain KFRI01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 374170..385537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9176_RS01875 (A9176_01855) - 374204..374743 (-) 540 WP_029510102.1 hypothetical protein -
  A9176_RS01880 (A9176_01860) adhP 374905..375960 (-) 1056 WP_029510101.1 alcohol dehydrogenase AdhP -
  A9176_RS01885 (A9176_01865) - 376123..377028 (-) 906 WP_077283218.1 NADP-dependent oxidoreductase -
  A9176_RS01890 (A9176_01870) gltX 377164..378660 (-) 1497 WP_077283220.1 glutamate--tRNA ligase -
  A9176_RS01895 (A9176_01875) - 378836..379159 (-) 324 WP_068851064.1 hypothetical protein -
  A9176_RS01900 (A9176_01880) radA 379170..380537 (-) 1368 WP_077281988.1 DNA repair protein RadA Machinery gene
  A9176_RS01905 (A9176_01885) - 380524..381405 (-) 882 WP_077281989.1 metallophosphoesterase family protein -
  A9176_RS01910 (A9176_01890) - 381640..382179 (-) 540 WP_077281990.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  A9176_RS01915 (A9176_01895) - 382405..382686 (+) 282 WP_010002509.1 GNAT family N-acetyltransferase -
  A9176_RS01920 (A9176_01900) rpiA 382683..383372 (+) 690 WP_029510095.1 ribose-5-phosphate isomerase RpiA -
  A9176_RS01925 (A9176_01905) - 383536..384960 (+) 1425 WP_077281991.1 aspartate ammonia-lyase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49227.40 Da        Isoelectric Point: 5.2659

>NTDB_id=186969 A9176_RS01900 WP_077281988.1 379170..380537(-) (radA) [Leuconostoc garlicum strain KFRI01]
MAKVKTQFICSNCGFTSARYLGRCSNCGEWGTFVEEKIMPETNDRKSRVSLDGRTAKVEKINEVTSEETPRVATNLKELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSGQLAHEGRVLYVTGEESATQVKLRADRLGVGNDEFYLYPETDMTAIKKQ
IESLQPDFVVIDSVQTMQEPDVTSAIGSVSQIREVTADLLQIAKTNNISIFIVGHVTKDGAIAGPKILEHMVDTVLYFEG
DSNYKYRILRAVKNRFGATNELGIFEMRDGGLIEVANPSEIFLEERLAGATGSAIVVALEGSRPILVELQALVTPTVFGN
AQRTASGLDRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVALASSYHDKESRPSDVFVGEIGLTGE
IRSVADIESRLKEARKLGFKRAIVPKNNLNGVTLPEGIQVIGVATLREALQLALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=186969 A9176_RS01900 WP_077281988.1 379170..380537(-) (radA) [Leuconostoc garlicum strain KFRI01]
ATCGCAAAAGTTAAAACACAATTTATTTGCTCAAACTGTGGTTTCACGTCAGCGCGCTATCTGGGTCGCTGCTCAAACTG
TGGTGAATGGGGCACATTTGTCGAAGAAAAAATCATGCCAGAGACCAACGACCGGAAAAGTCGGGTGAGTCTAGACGGTC
GTACGGCTAAGGTTGAAAAGATTAATGAGGTGACATCAGAGGAAACACCGCGTGTTGCCACGAACCTTAAAGAACTGAAC
CGCGTGTTAGGCGGTGGGGTGGTTCCAGGTTCAATGGTGTTGATTGGTGGGGATCCAGGCATTGGGAAATCAACCCTCTT
GCTCCAAGTCTCTGGGCAATTGGCCCATGAAGGTCGTGTGTTATACGTGACTGGGGAAGAATCTGCCACCCAAGTGAAGC
TACGGGCAGATCGTTTGGGTGTTGGGAATGATGAGTTTTACCTCTATCCAGAAACAGATATGACGGCGATTAAAAAGCAG
ATTGAATCGCTGCAACCAGATTTTGTCGTGATTGACTCAGTACAAACGATGCAGGAACCAGACGTGACGTCCGCCATTGG
CTCCGTGTCACAAATCCGCGAAGTCACGGCAGATTTATTGCAAATTGCTAAAACCAATAACATTTCTATTTTTATCGTTG
GTCACGTGACTAAAGATGGGGCGATTGCTGGACCAAAGATTTTGGAGCATATGGTCGATACGGTGCTCTATTTTGAAGGT
GATAGTAACTATAAGTACCGTATTTTACGTGCGGTGAAAAATCGTTTTGGCGCCACCAATGAATTGGGTATTTTTGAAAT
GCGTGACGGCGGCTTGATTGAAGTCGCTAACCCATCAGAAATCTTTTTGGAAGAACGGTTAGCTGGGGCAACTGGGTCGG
CCATTGTGGTCGCCTTGGAAGGCTCGCGGCCGATTTTGGTTGAATTACAGGCTTTAGTGACGCCCACCGTCTTTGGCAAT
GCGCAACGGACGGCATCCGGATTAGATCGTAACCGCGTGTCGTTAATTATGGCCGTTTTGGAAAAGCGCGCCAATCTCTT
GTTGCAAAATCAAGATGCTTATCTGAAAGCTGCCGGTGGGGTGAAACTGGATGAACCCGCAATTGATTTGGCGATTGCCG
TGGCACTGGCCAGTTCTTATCACGATAAAGAATCGCGGCCAAGTGACGTCTTTGTCGGTGAAATTGGGTTAACTGGGGAA
ATCCGGAGCGTCGCTGATATTGAAAGTCGGTTGAAGGAGGCGCGCAAGCTTGGCTTTAAACGGGCGATTGTACCAAAGAA
CAACTTGAATGGGGTCACACTTCCTGAAGGGATTCAAGTAATTGGCGTCGCCACGTTACGAGAAGCGTTGCAATTAGCCT
TAGATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(362-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B8TEG9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

66.962

99.121

0.664
  radA Streptococcus pneumoniae D39

66.962

99.121

0.664
  radA Streptococcus pneumoniae R6

66.962

99.121

0.664
  radA Streptococcus pneumoniae TIGR4

66.962

99.121

0.664
  radA Streptococcus mitis NCTC 12261

66.741

99.121

0.662
  radA Streptococcus mitis SK321

66.741

99.121

0.662
  radA Bacillus subtilis subsp. subtilis str. 168

62.281

100

0.624

Multiple sequence alignment