Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BA203_RS00535 Genome accession   NZ_CP016315
Coordinates   97717..99093 (+) Length   458 a.a.
NCBI ID   WP_088859339.1    Uniprot ID   -
Organism   Bacillus cereus strain D12_2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92717..104093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BA203_RS00515 (BA203_00510) ctsR 92914..93375 (+) 462 WP_001244558.1 transcriptional regulator CtsR -
  BA203_RS00520 (BA203_00515) - 93546..94094 (+) 549 WP_000128399.1 UvrB/UvrC motif-containing protein -
  BA203_RS00525 (BA203_00520) - 94099..95163 (+) 1065 WP_000050843.1 protein arginine kinase -
  BA203_RS00530 (BA203_00525) clpC 95186..97621 (+) 2436 WP_000971184.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  BA203_RS00535 (BA203_00530) radA 97717..99093 (+) 1377 WP_088859339.1 DNA repair protein RadA Machinery gene
  BA203_RS00540 (BA203_00535) disA 99097..100170 (+) 1074 WP_000392158.1 DNA integrity scanning diadenylate cyclase DisA -
  BA203_RS00545 (BA203_00540) - 100332..101441 (+) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  BA203_RS00550 (BA203_00545) ispD 101458..102138 (+) 681 WP_000288283.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BA203_RS00555 (BA203_00550) ispF 102255..102731 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49871.50 Da        Isoelectric Point: 7.1422

>NTDB_id=186803 BA203_RS00535 WP_088859339.1 97717..99093(+) (radA) [Bacillus cereus strain D12_2]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGNNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=186803 BA203_RS00535 WP_088859339.1 97717..99093(+) (radA) [Bacillus cereus strain D12_2]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAATAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAAGTGACATCAGC
TCCTGGTAGTGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTTA
TCGTAGGGCATGTGACAAAAGAAGGTGCAATTGCGGGACCGCGTATGCTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629

Multiple sequence alignment