Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AOT18_RS16545 Genome accession   NZ_CP016300
Coordinates   3409884..3410684 (-) Length   266 a.a.
NCBI ID   WP_002027454.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain CMC-CR-MDR-Ab66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3404884..3415684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOT18_RS16525 (AOT18_16235) - 3405949..3406647 (-) 699 WP_000058788.1 YfgM family protein -
  AOT18_RS16530 (AOT18_16240) hisS 3406676..3407968 (-) 1293 WP_000095264.1 histidine--tRNA ligase -
  AOT18_RS16535 (AOT18_16245) ispG 3407965..3409080 (-) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  AOT18_RS16540 (AOT18_16250) - 3409102..3409893 (-) 792 WP_000405164.1 helix-turn-helix domain-containing protein -
  AOT18_RS16545 (AOT18_16255) pilF 3409884..3410684 (-) 801 WP_002027454.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  AOT18_RS16550 (AOT18_16260) rlmN 3410700..3411932 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  AOT18_RS16555 (AOT18_16265) ndk 3412055..3412486 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  AOT18_RS16560 (AOT18_16270) iscX 3412600..3412800 (-) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  AOT18_RS16565 (AOT18_16275) - 3412900..3413451 (-) 552 WP_000669567.1 phosphatase PAP2 family protein -
  AOT18_RS16570 (AOT18_16280) - 3413476..3414774 (-) 1299 WP_000348500.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30124.47 Da        Isoelectric Point: 9.2307

>NTDB_id=186701 AOT18_RS16545 WP_002027454.1 3409884..3410684(-) (pilF) [Acinetobacter baumannii strain CMC-CR-MDR-Ab66]
MSTPKLKIMLCMGVAVALLTSGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=186701 AOT18_RS16545 WP_002027454.1 3409884..3410684(-) (pilF) [Acinetobacter baumannii strain CMC-CR-MDR-Ab66]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGACAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAACAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCATCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

99.624

100

0.996
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726

Multiple sequence alignment