Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   A4W79_RS03890 Genome accession   NZ_CP016221
Coordinates   743129..743800 (+) Length   223 a.a.
NCBI ID   WP_004270751.1    Uniprot ID   A0A1B2A7J9
Organism   Latilactobacillus curvatus strain TMW 1.421     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 738129..748800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W79_RS03860 (A4W79_03815) - 738340..739527 (+) 1188 WP_004270744.1 FtsW/RodA/SpoVE family cell cycle protein -
  A4W79_RS03865 (A4W79_03820) - 739540..740628 (+) 1089 WP_065825727.1 CAP-associated domain-containing protein -
  A4W79_RS03870 (A4W79_03825) - 740648..740938 (+) 291 WP_004270748.1 YlbG family protein -
  A4W79_RS03875 (A4W79_03830) rsmD 740944..741498 (+) 555 WP_004270750.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  A4W79_RS03880 (A4W79_03835) coaD 741510..742004 (+) 495 WP_065825725.1 pantetheine-phosphate adenylyltransferase -
  A4W79_RS03885 (A4W79_03840) - 742006..743052 (+) 1047 WP_253726877.1 SepM family pheromone-processing serine protease -
  A4W79_RS03890 (A4W79_03845) comEA 743129..743800 (+) 672 WP_004270751.1 helix-hairpin-helix domain-containing protein Machinery gene
  A4W79_RS03895 (A4W79_03850) - 743859..744347 (+) 489 WP_004270740.1 ComE operon protein 2 -
  A4W79_RS03900 (A4W79_03855) comEC 744488..746602 (+) 2115 WP_223315707.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  A4W79_RS03905 (A4W79_03860) holA 746603..747631 (+) 1029 WP_089542075.1 DNA polymerase III subunit delta -
  A4W79_RS03910 (A4W79_03865) rpsT 747687..747941 (-) 255 WP_004271117.1 30S ribosomal protein S20 -
  A4W79_RS03915 (A4W79_03870) rpsO 748218..748487 (+) 270 WP_004271112.1 30S ribosomal protein S15 -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24849.51 Da        Isoelectric Point: 9.9048

>NTDB_id=186316 A4W79_RS03890 WP_004270751.1 743129..743800(+) (comEA) [Latilactobacillus curvatus strain TMW 1.421]
MWRDYWQRYRRFWWVPCLVIILLGGGVFWQHSRQQRFLSTNQQLMAQSTKRKVKAKPVKQKKSVSKGGFVDIKGAVAHPG
IYPICVAKTRLFEVLKQAGDVTEAADTSQLNLAQTLSDQLIIYVPTKGEAVQAEELVKAADEPQAATDNQAGASPISTDA
KINLNQADATQLQTLNGIGPKKAEQIIAYRDEQGQFKQIEDLQKVGGIGPKTFEQLRPQICVN

Nucleotide


Download         Length: 672 bp        

>NTDB_id=186316 A4W79_RS03890 WP_004270751.1 743129..743800(+) (comEA) [Latilactobacillus curvatus strain TMW 1.421]
ATGTGGCGCGACTATTGGCAACGGTATCGTCGATTCTGGTGGGTACCTTGTTTGGTGATAATTTTACTTGGTGGTGGTGT
GTTTTGGCAGCATAGTCGGCAACAACGTTTTCTATCAACCAATCAACAATTGATGGCGCAATCAACTAAGCGAAAAGTGA
AGGCAAAACCCGTCAAACAGAAGAAAAGCGTCTCAAAAGGTGGGTTTGTCGATATCAAAGGCGCTGTGGCACATCCAGGG
ATTTATCCGATTTGTGTTGCAAAGACGCGTTTATTTGAGGTGCTGAAACAAGCGGGGGATGTGACCGAAGCAGCGGATAC
GAGTCAGTTGAACTTAGCGCAAACATTGAGTGACCAACTTATTATCTATGTGCCAACTAAGGGAGAAGCGGTGCAAGCCG
AAGAATTAGTGAAAGCGGCAGACGAGCCACAGGCTGCGACTGACAATCAAGCGGGAGCCAGTCCGATATCAACTGATGCT
AAAATCAACCTAAACCAAGCGGATGCGACACAATTGCAAACGCTAAATGGCATCGGTCCTAAAAAAGCTGAACAAATAAT
TGCATATCGGGATGAGCAAGGGCAGTTTAAACAAATCGAAGATCTTCAAAAAGTTGGGGGAATTGGGCCGAAAACGTTTG
AACAATTACGGCCACAAATTTGTGTTAATTAG

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-115)

Helix-hairpin-helix motif (HHH_3)

(160-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B2A7J9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Latilactobacillus sakei subsp. sakei 23K

64.53

100

0.677
  comEA Lactococcus lactis subsp. cremoris KW2

36.283

100

0.368

Multiple sequence alignment