Detailed information NTG0186312

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: A6J30_RS07775
• Genome accession: NZ_CP020427
• Coordinates: 1603810..1606014 (+)
• Length: 734 a.a.
• NCBI ID: WP_005481427.1
• Uniprot ID: Q87T17
• Organism: Vibrio parahaemolyticus strain FDAARGOS_191
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1598810..1611014

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6J30_RS07730 (A6J30_07475)	rplV	1599479..1599811 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
A6J30_RS07735 (A6J30_07480)	rpsS	1599822..1600100 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
A6J30_RS07740 (A6J30_07485)	rplB	1600122..1600946 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
A6J30_RS07745 (A6J30_07490)	rplW	1600962..1601264 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
A6J30_RS07750 (A6J30_07495)	rplD	1601261..1601863 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
A6J30_RS07755 (A6J30_07500)	rplC	1601881..1602510 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
A6J30_RS07760 (A6J30_07505)	rpsJ	1602525..1602836 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
A6J30_RS07765 (A6J30_07510)	rpmE	1603294..1603515 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
A6J30_RS07775 (A6J30_07515)	priA	1603810..1606014 (+)	2205	WP_005481427.1	primosomal protein N'	Machinery gene
A6J30_RS07780 (A6J30_07520)	cytR	1606347..1607354 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
A6J30_RS07785 (A6J30_07525)	ftsN	1607531..1608076 (+)	546	WP_005481405.1	cell division protein FtsN	-
A6J30_RS07790 (A6J30_07530)	hslV	1608241..1608792 (+)	552	WP_005455873.1	ATP-dependent protease subunit HslV	-
A6J30_RS07795 (A6J30_07535)	hslU	1608816..1610147 (+)	1332	WP_005457198.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82271.83 Da
• Isoelectric Point: 8.5714

>NTDB_id=186312 A6J30_RS07775 WP_005481427.1 1603810..1606014(+) (priA) [Vibrio parahaemolyticus strain FDAARGOS_191]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLIWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAIKQQKVLQMLVNGALPHQEFVDQ
EITSTVLKSLEDKGWIERIEKKPAITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTSNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLIQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=186312 A6J30_RS07775 WP_005481427.1 1603810..1606014(+) (priA) [Vibrio parahaemolyticus strain FDAARGOS_191]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGATTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGATCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAACCTCAACTGTACTAAAGTCTTTAGAAGATAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGCAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAACGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGATCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCCCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTTCTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTGGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGAGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTGATAGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAACACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCTCACCCTTTGTT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAA
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• DEAD/DEAH box helicase (DEAD) (220-365)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

3D structure

• AlphaFold DB: Q87T17

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.649	100	0.44