Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A9255_RS17250 Genome accession   NZ_CP016176
Coordinates   3788633..3790066 (-) Length   477 a.a.
NCBI ID   WP_069317790.1    Uniprot ID   A0A2G0QFY3
Organism   Xenorhabdus hominickii strain ANU1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783633..3795066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9255_RS17215 (A9255_17215) rnk 3784161..3784571 (-) 411 WP_069317783.1 nucleoside diphosphate kinase regulator -
  A9255_RS17220 (A9255_17220) alr 3784776..3786008 (-) 1233 WP_069317784.1 alanine racemase -
  A9255_RS17225 (A9255_17225) - 3786219..3786443 (+) 225 WP_145957428.1 hypothetical protein -
  A9255_RS17230 (A9255_17230) npr 3786472..3786744 (-) 273 WP_069317786.1 PTS phosphocarrier protein NPr -
  A9255_RS17235 (A9255_17235) rapZ 3786777..3787628 (-) 852 WP_069317787.1 RNase adapter RapZ -
  A9255_RS17240 (A9255_17240) ptsN 3787667..3788134 (-) 468 WP_069317788.1 PTS IIA-like nitrogen regulatory protein PtsN -
  A9255_RS17245 (A9255_17245) hpf 3788322..3788609 (-) 288 WP_069317789.1 ribosome hibernation promoting factor -
  A9255_RS17250 (A9255_17250) rpoN/rpoN1 3788633..3790066 (-) 1434 WP_069317790.1 RNA polymerase factor sigma-54 Machinery gene
  A9255_RS17255 (A9255_17255) lptB 3790146..3790871 (-) 726 WP_069317791.1 LPS export ABC transporter ATP-binding protein -
  A9255_RS17260 (A9255_17260) lptA 3790878..3791426 (-) 549 WP_069317792.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  A9255_RS17265 (A9255_17265) lptC 3791407..3792000 (-) 594 WP_069317793.1 LPS export ABC transporter periplasmic protein LptC -
  A9255_RS17270 (A9255_17270) kdsC 3792019..3792582 (-) 564 WP_069317794.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  A9255_RS17275 (A9255_17275) kdsD 3792634..3793602 (-) 969 WP_069317795.1 arabinose-5-phosphate isomerase KdsD -
  A9255_RS17280 (A9255_17280) - 3793638..3794615 (-) 978 WP_069317796.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54030.32 Da        Isoelectric Point: 4.4533

>NTDB_id=186063 A9255_RS17250 WP_069317790.1 3788633..3790066(-) (rpoN/rpoN1) [Xenorhabdus hominickii strain ANU1]
MKQSLQLKLSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALDTNPLLEQEDPHEEVDSQEPPDTEVMDAREALEQKEMPE
ELPLDTSWDEIYSAGTLSGTSSDYKTEDFPVYQGETHQTLQDYLMWQAELTPFTETDFAIATSIIDAIDDTGYLTVSVDE
ILISIGNDELALVEVETVLKRIQHFDPIGVAARDLRECLLIQLSILLPETPHLNEAKLIVSKYLELLGNRDFRNLLRQSK
LKENALKGAIDIIQSLEPKPGLVVNTSESEYVIPDVLVQKVNESWQVRLNADSIPRLRINQQYAAMGQQTNNENDSLFIR
SNLQEAKWLIKSLESRNETLLKVAICIVERQQDFFEHGEEYMKPLVLADIAYQIDMHESTISRVTTQKYLYSPRGIFELK
YFFSSHVNTDSGGEASSTAIRALVKKMVAAENPAKPLSDSKLTSILAEQGIQVARRTVAKYRESLSIPPSNQRKKLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=186063 A9255_RS17250 WP_069317790.1 3788633..3790066(-) (rpoN/rpoN1) [Xenorhabdus hominickii strain ANU1]
ATGAAGCAAAGTTTGCAACTCAAGCTCAGTCAGCAATTGGCAATGACGCCACAACTCCAGCAAGCTATCCGTTTGTTGCA
ACTTTCTACGCTTGAACTTCAACAAGAAATTCAGCTTGCTTTGGACACCAATCCTCTGCTTGAACAAGAGGATCCACATG
AAGAAGTGGATAGCCAGGAACCCCCTGATACTGAAGTGATGGACGCCCGAGAAGCGCTAGAACAGAAAGAGATGCCTGAA
GAACTCCCACTGGATACCAGTTGGGATGAAATTTACTCCGCTGGCACGCTTTCAGGAACAAGCAGTGATTACAAAACTGA
GGACTTTCCCGTCTATCAGGGAGAGACACATCAGACGCTGCAAGATTATCTGATGTGGCAGGCAGAATTGACGCCGTTTA
CCGAAACCGATTTTGCCATTGCCACGTCAATCATCGATGCTATTGATGACACAGGCTATCTGACTGTTTCTGTCGACGAA
ATCCTTATCAGTATCGGTAATGACGAACTGGCGTTGGTAGAAGTCGAAACCGTTCTCAAGCGCATACAGCATTTTGATCC
GATTGGTGTGGCTGCCCGTGATTTGCGGGAATGCTTGCTTATCCAGCTTTCAATTTTGCTACCAGAAACTCCACATCTGA
ACGAAGCCAAACTCATTGTCAGCAAATATCTTGAACTGCTGGGCAATCGAGACTTCCGTAATTTATTACGCCAGAGCAAA
TTAAAAGAGAACGCTCTGAAAGGAGCGATTGATATTATTCAATCACTGGAGCCGAAACCGGGGTTGGTAGTCAATACCAG
TGAATCCGAATATGTTATTCCCGATGTCTTAGTGCAGAAAGTCAATGAAAGCTGGCAAGTCAGGCTAAATGCCGATAGCA
TTCCCCGTCTGCGTATCAACCAGCAATATGCAGCAATGGGGCAACAGACCAATAATGAAAATGACAGTCTGTTCATCCGC
AGTAATTTGCAGGAAGCCAAGTGGCTGATAAAAAGCTTAGAAAGCCGCAATGAGACATTATTGAAAGTCGCCATCTGCAT
TGTTGAACGGCAACAAGACTTTTTTGAACATGGTGAGGAGTACATGAAACCGCTGGTATTGGCTGATATTGCCTATCAGA
TTGACATGCATGAATCCACCATCTCCCGCGTAACGACACAGAAATACCTGTATAGTCCACGAGGGATTTTTGAACTTAAG
TATTTTTTCTCCAGTCACGTCAATACAGATAGTGGAGGTGAAGCCTCTTCTACCGCAATACGTGCGCTGGTGAAAAAAAT
GGTCGCGGCAGAAAACCCGGCTAAGCCACTGAGTGATAGTAAATTGACCAGTATACTGGCTGAACAAGGTATTCAAGTGG
CACGTCGGACTGTCGCAAAATATAGAGAGTCGTTATCCATCCCGCCTTCAAACCAACGTAAAAAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G0QFY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482

Multiple sequence alignment