Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AL025_RS02730 Genome accession   NZ_CP016163
Coordinates   533679..535049 (+) Length   456 a.a.
NCBI ID   WP_002287449.1    Uniprot ID   A0A132Z2P0
Organism   Enterococcus faecium strain ISMMS_VRE_11     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 528679..540049
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL025_RS02710 (AL025_02525) - 529026..530273 (-) 1248 WP_002287452.1 glutamate-5-semialdehyde dehydrogenase -
  AL025_RS02715 (AL025_02530) proB 530270..531076 (-) 807 WP_002287451.1 glutamate 5-kinase -
  AL025_RS02720 (AL025_02535) - 531429..532679 (-) 1251 WP_002287107.1 IS110 family transposase -
  AL025_RS02725 (AL025_02540) - 533089..533616 (+) 528 WP_002287450.1 dUTP diphosphatase -
  AL025_RS02730 (AL025_02545) radA 533679..535049 (+) 1371 WP_002287449.1 DNA repair protein RadA Machinery gene
  AL025_RS02735 (AL025_02550) - 535083..536240 (+) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  AL025_RS02740 (AL025_02555) gltX 536410..537870 (+) 1461 WP_002287447.1 glutamate--tRNA ligase -
  AL025_RS02745 (AL025_02560) epsC 538192..538728 (+) 537 WP_002287446.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49949.63 Da        Isoelectric Point: 7.7416

>NTDB_id=185911 AL025_RS02730 WP_002287449.1 533679..535049(+) (radA) [Enterococcus faecium strain ISMMS_VRE_11]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=185911 AL025_RS02730 WP_002287449.1 533679..535049(+) (radA) [Enterococcus faecium strain ISMMS_VRE_11]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACGGACCGCAGAACTCGGACAAGTTTGACAGGAG
AAAAAGCAAAACCAACAAAAATCGCAGACGTCGTCCCTAAAAAAGAACCTCGGATCAAAACAAAATTAGAAGAACTGAAC
CGTGTGTTAGGCGGAGGTGTGGTGCCAGGGTCTATGGTCTTGATTGGTGGCGATCCAGGTATCGGGAAATCCACTTTGCT
GTTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGAAAAGTCTTATATGTATCCGGCGAAGAAAGCGCAGAGCAGATAA
AATTACGCGCGGAACGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAACGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATCATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGTGTCAGCCAAGTAAGAGAAACCACTGCAGAACTACTCAAAATCGCTAAAACAAATGGGATAGCCATTTTTATCG
TCGGTCATGTTACCAAAGAAGGCTCGATCGCCGGCCCAAGAATGTTAGAACATATGGTGGATACGGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTTCGGATTTTGCGAGCAGTAAAAAACCGATTTGGCTCAACGAATGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAATCCTTCACAGATTTTCTTAGAAGAACGCCTAGACGGTGCAACTGGCT
CTGCTATCGTTGTGGCAATGGAAGGGACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCCAAACGAACAACAACAGGTCTTGATTTCAATCGGGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGGTT
GCTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTATAAAGAAAAAGGAACACAGCCAACGGAATGTTTCATCGGTGAAATTGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGAGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTATACCTACCTAA
AAACAACTTAGGTAACTGGGAAGCGCCAAAAGGAATCGAGATAGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A132Z2P0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.286

99.781

0.741
  radA Streptococcus pneumoniae Rx1

74.286

99.781

0.741
  radA Streptococcus pneumoniae D39

74.286

99.781

0.741
  radA Streptococcus pneumoniae R6

74.286

99.781

0.741
  radA Streptococcus pneumoniae TIGR4

74.286

99.781

0.741
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667

Multiple sequence alignment