Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   A9I65_RS06415 Genome accession   NZ_CP016073
Coordinates   1367135..1367659 (+) Length   174 a.a.
NCBI ID   WP_256726570.1    Uniprot ID   -
Organism   Staphylococcus pseudintermedius strain 081661 isolate 20081661     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1362135..1372659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9I65_RS06385 (A9I65_06385) - 1362382..1362729 (-) 348 WP_015729174.1 hypothetical protein -
  A9I65_RS13255 - 1362726..1362878 (-) 153 WP_015729173.1 ribbon-helix-helix protein, CopG family -
  A9I65_RS06390 (A9I65_06390) - 1363008..1363670 (+) 663 WP_015729172.1 hypothetical protein -
  A9I65_RS06395 (A9I65_06395) - 1363660..1364022 (+) 363 WP_037542934.1 hypothetical protein -
  A9I65_RS06400 (A9I65_06400) - 1364082..1365233 (+) 1152 WP_015729170.1 SNF2-related protein -
  A9I65_RS06405 (A9I65_06405) - 1365233..1366522 (+) 1290 WP_049770165.1 DUF3440 domain-containing protein -
  A9I65_RS06410 (A9I65_06410) - 1366516..1367022 (+) 507 WP_198463136.1 ParB/RepB/Spo0J family partition protein -
  A9I65_RS06415 (A9I65_06415) comGA 1367135..1367659 (+) 525 WP_256726570.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  A9I65_RS06420 (A9I65_06420) comGB 1367628..1368695 (+) 1068 WP_037542939.1 competence type IV pilus assembly protein ComGB -
  A9I65_RS06425 (A9I65_06425) comGC 1368708..1369031 (+) 324 WP_015729165.1 competence type IV pilus major pilin ComGC Machinery gene
  A9I65_RS06430 (A9I65_06430) comGD 1369009..1369449 (+) 441 WP_014613823.1 competence type IV pilus minor pilin ComGD -
  A9I65_RS13260 - 1369554..1369754 (+) 201 WP_020219623.1 hypothetical protein -
  A9I65_RS06435 (A9I65_06435) - 1369660..1370145 (+) 486 WP_081378286.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  A9I65_RS06445 (A9I65_06445) - 1370309..1370836 (+) 528 WP_014613826.1 shikimate kinase -
  A9I65_RS06450 (A9I65_06450) gcvT 1370994..1372085 (+) 1092 WP_015729161.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 19960.92 Da        Isoelectric Point: 6.3729

>NTDB_id=185669 A9I65_RS06415 WP_256726570.1 1367135..1367659(+) (comGA) [Staphylococcus pseudintermedius strain 081661 isolate 20081661]
MFLYTYTHVYITFQYPVEQLVNGITQISVNEKADITYGSSLKAILRCDPDVILIGEIRDSHIARDVIQASLSGHLVLSTI
HANDCQGVLLRLIEMGLSHQDLSQSINLIINQRLITTKNDQRQLVYETMDQQEIHYFLNNNFQLPTAFTSLSHKLQLLNK
EGVIDEKILRKYTI

Nucleotide


Download         Length: 525 bp        

>NTDB_id=185669 A9I65_RS06415 WP_256726570.1 1367135..1367659(+) (comGA) [Staphylococcus pseudintermedius strain 081661 isolate 20081661]
ATGTTTTTGTACACCTATACACACGTATACATCACATTCCAGTATCCGGTCGAGCAGCTTGTGAACGGCATTACGCAAAT
TTCCGTTAACGAAAAAGCTGATATTACGTATGGATCGTCGTTAAAAGCAATTCTTCGTTGTGATCCAGATGTCATCTTAA
TTGGTGAGATTCGTGACAGTCATATTGCAAGGGACGTTATACAAGCGAGTTTAAGTGGGCATTTAGTATTGTCGACGATA
CACGCCAATGATTGTCAAGGGGTGCTCCTTAGACTGATTGAAATGGGACTCTCTCACCAAGATTTATCTCAATCTATCAA
TCTCATCATCAATCAAAGACTCATTACAACAAAAAACGACCAGCGACAACTCGTCTATGAAACGATGGATCAACAGGAGA
TTCATTACTTTTTGAATAATAACTTCCAACTACCTACTGCTTTCACAAGCCTTTCTCATAAGCTTCAACTTCTGAATAAA
GAGGGGGTTATAGATGAAAAGATTTTACGAAAGTATACGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

59.259

93.103

0.552
  comGA Staphylococcus aureus N315

59.259

93.103

0.552

Multiple sequence alignment