Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   A9404_RS12625 Genome accession   NZ_CP016027
Coordinates   2821022..2822404 (-) Length   460 a.a.
NCBI ID   WP_066102278.1    Uniprot ID   A0A191ZJS5
Organism   Halothiobacillus diazotrophicus strain LS2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2816022..2827404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9404_RS12600 (A9404_12600) - 2816573..2817622 (+) 1050 WP_066102263.1 RnfABCDGE type electron transport complex subunit D -
  A9404_RS12605 (A9404_12605) - 2817619..2818368 (+) 750 WP_066102265.1 RnfABCDGE type electron transport complex subunit G -
  A9404_RS12610 (A9404_12610) - 2818361..2819080 (+) 720 WP_066102269.1 electron transport complex subunit E -
  A9404_RS12615 (A9404_12615) - 2819115..2819543 (-) 429 WP_066102273.1 hypothetical protein -
  A9404_RS13685 - 2819771..2819902 (+) 132 WP_257736801.1 hypothetical protein -
  A9404_RS12620 (A9404_12620) - 2820040..2820906 (-) 867 WP_066102275.1 GyrI-like domain-containing protein -
  A9404_RS12625 (A9404_12625) radA 2821022..2822404 (-) 1383 WP_066102278.1 DNA repair protein RadA Machinery gene
  A9404_RS12630 (A9404_12630) - 2822404..2823675 (-) 1272 WP_066102281.1 HlyC/CorC family transporter -
  A9404_RS12635 (A9404_12635) flgB 2823880..2824287 (+) 408 WP_082922973.1 flagellar basal body rod protein FlgB -
  A9404_RS12640 (A9404_12640) flgC 2824300..2824701 (+) 402 WP_066102287.1 flagellar basal body rod protein FlgC -
  A9404_RS12645 (A9404_12645) - 2824714..2825433 (+) 720 WP_066102290.1 flagellar hook assembly protein FlgD -
  A9404_RS12650 (A9404_12650) flgE 2825488..2826741 (+) 1254 WP_066102293.1 flagellar hook protein FlgE -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 48912.31 Da        Isoelectric Point: 7.2817

>NTDB_id=185435 A9404_RS12625 WP_066102278.1 2821022..2822404(-) (radA) [Halothiobacillus diazotrophicus strain LS2]
MARAKEKSAFVCQECGADFPKWAGRCSECGAWNSLTEIRIPNAPAGARNERAAGYAGGSPQVTILSEVRISETPRETTGL
DELDRVLGGGLVPGSVVLIGGDPGIGKSTLLLQTLLTLQANMPCLYVTGEESLQQVALRAQRLGVDLAGKEGQLTLMSET
SVESILAHCARQLPRLLVVDSIQTLHTEGLTAAPGSVSQLREGTAQLVRFAKRHDVTIFIVGHVTKEGALAGPRVLEHMV
DTVLYFEGDPGARFRVIRAVKNRFGAANEIGVFAMTDRGLKPVSNPSAIFLSRHEQPVAGSVITVTREGTRPLLVEVQAL
VAEHPGGNPRRLTVGLEQNRLAMLLAVLHRHGQIALYDQDVFLNVVGGVRVGETAADLPSLLAVLSSFRNRPLPEGLVVF
GEVGLAGEIRPVSGGPERLAEAAKHGMTHAIVPKANLPGRPIDGLTITPVSHLNQALDLI

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=185435 A9404_RS12625 WP_066102278.1 2821022..2822404(-) (radA) [Halothiobacillus diazotrophicus strain LS2]
ATGGCGCGGGCGAAGGAAAAATCCGCCTTCGTCTGCCAGGAATGCGGTGCCGACTTCCCCAAATGGGCGGGACGCTGCAG
CGAATGCGGGGCCTGGAACAGTCTCACGGAAATTCGTATACCCAATGCGCCCGCCGGCGCACGCAACGAGCGTGCGGCCG
GCTATGCCGGCGGCAGTCCCCAGGTCACGATCCTGTCGGAGGTGCGCATCAGCGAAACGCCCCGGGAGACGACCGGCCTC
GACGAACTCGACCGCGTCCTGGGTGGCGGCCTCGTTCCGGGGTCGGTCGTCCTGATCGGCGGCGATCCCGGCATCGGCAA
ATCCACCCTGCTGCTGCAAACCCTGCTGACCCTGCAGGCGAACATGCCCTGCCTGTACGTCACCGGCGAGGAATCCCTCC
AGCAGGTCGCCCTGCGGGCACAGCGCCTGGGGGTCGATCTCGCGGGCAAGGAAGGCCAGCTCACGCTGATGAGCGAAACC
TCGGTCGAATCGATCCTTGCCCATTGCGCCCGGCAACTGCCACGCCTGCTCGTCGTGGATTCCATCCAGACCCTGCATAC
GGAAGGGCTCACCGCCGCGCCCGGCTCGGTATCCCAGCTGCGCGAAGGCACGGCCCAACTGGTCCGCTTCGCCAAGCGCC
ACGACGTCACGATCTTCATCGTGGGTCACGTCACCAAGGAAGGCGCCTTGGCCGGTCCGCGCGTGCTGGAGCATATGGTC
GATACCGTCCTGTACTTCGAAGGCGATCCGGGCGCGCGTTTCCGCGTGATCCGGGCCGTCAAGAACCGCTTCGGCGCCGC
CAACGAGATCGGCGTCTTCGCGATGACGGACCGTGGCCTCAAACCCGTCAGCAATCCGTCCGCCATTTTTTTGTCACGTC
ATGAGCAACCTGTCGCGGGCAGCGTGATCACCGTGACGCGCGAGGGCACGCGCCCCCTGCTTGTCGAAGTGCAGGCACTG
GTAGCCGAGCACCCGGGCGGCAATCCCCGGCGGCTCACGGTCGGCCTGGAGCAGAACCGTCTGGCGATGCTGCTGGCCGT
GCTGCATCGGCACGGCCAGATCGCCCTCTACGATCAAGATGTCTTTCTGAATGTGGTCGGCGGCGTGCGCGTCGGCGAAA
CCGCGGCGGATCTACCCAGCCTGCTCGCCGTGCTGTCGAGCTTCCGAAACCGCCCGCTGCCCGAGGGACTCGTCGTCTTC
GGCGAAGTCGGGCTTGCTGGCGAGATTCGCCCGGTCAGCGGCGGTCCGGAACGCTTGGCCGAAGCCGCCAAGCACGGGAT
GACCCATGCCATCGTCCCCAAGGCCAACCTGCCGGGTCGACCGATCGACGGCTTGACCATCACGCCCGTCAGCCACCTCA
ATCAAGCACTGGACCTTATCTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

Subunit ChlI of Mg-chelatase (ChlI)

(369-436)

KaiC (ATPase)

(78-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZJS5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.783

100

0.498
  radA Streptococcus pneumoniae Rx1

49.083

94.783

0.465
  radA Streptococcus pneumoniae D39

49.083

94.783

0.465
  radA Streptococcus pneumoniae R6

49.083

94.783

0.465
  radA Streptococcus pneumoniae TIGR4

49.083

94.783

0.465
  radA Streptococcus mitis SK321

49.083

94.783

0.465
  radA Streptococcus mitis NCTC 12261

48.853

94.783

0.463

Multiple sequence alignment