Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A9Y76_RS01675 Genome accession   NZ_CP016022
Coordinates   333947..335455 (-) Length   502 a.a.
NCBI ID   WP_064801480.1    Uniprot ID   A0A191ZT41
Organism   Ralstonia insidiosa strain ATCC 49129     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328947..340455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9Y76_RS01645 (A9Y76_01645) - 329404..330057 (-) 654 WP_031329820.1 LON peptidase substrate-binding domain-containing protein -
  A9Y76_RS01650 (A9Y76_01650) rapZ 330156..331058 (-) 903 WP_021196397.1 RNase adapter RapZ -
  A9Y76_RS01655 (A9Y76_01655) - 331152..331505 (-) 354 WP_049285390.1 PsiF family protein -
  A9Y76_RS01660 (A9Y76_01660) hprK 331692..332666 (-) 975 WP_021196399.1 HPr(Ser) kinase/phosphatase -
  A9Y76_RS01665 (A9Y76_01665) ptsN 332740..333195 (-) 456 WP_021196400.1 PTS IIA-like nitrogen regulatory protein PtsN -
  A9Y76_RS01670 (A9Y76_01670) hpf 333542..333895 (-) 354 WP_021196401.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A9Y76_RS01675 (A9Y76_01675) rpoN/rpoN1 333947..335455 (-) 1509 WP_064801480.1 RNA polymerase factor sigma-54 Machinery gene
  A9Y76_RS01680 (A9Y76_01680) lptB 335587..336375 (-) 789 WP_021196403.1 LPS export ABC transporter ATP-binding protein -
  A9Y76_RS01685 (A9Y76_01685) lptA 336404..336985 (-) 582 WP_064801482.1 lipopolysaccharide transport periplasmic protein LptA -
  A9Y76_RS01690 (A9Y76_01690) lptC 337059..337691 (-) 633 WP_064801489.1 LPS export ABC transporter periplasmic protein LptC -
  A9Y76_RS01695 (A9Y76_01695) - 337696..338307 (-) 612 WP_064801491.1 KdsC family phosphatase -
  A9Y76_RS01700 (A9Y76_01700) - 338324..339307 (-) 984 WP_064806139.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 55663.00 Da        Isoelectric Point: 4.8484

>NTDB_id=185338 A9Y76_RS01675 WP_064801480.1 333947..335455(-) (rpoN/rpoN1) [Ralstonia insidiosa strain ATCC 49129]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQNNNATPLPTE
SPPPSPNGADSSDTPDNSYDGHDSGADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPLQLREAEQSLREFLMEQLAPLKL
SMRDKGLVIFLIESLDDEGYLSATLDEVLTDLPAELEVELDELQAALRMLQSFDPPGVGARSAAECLSLQLHRLDSPAKA
LALIIVNQHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTSAGWMAQLNPDVM
PRLRINDMYAQILRSSRGETGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTL
GLHESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=185338 A9Y76_RS01675 WP_064801480.1 333947..335455(-) (rpoN/rpoN1) [Ralstonia insidiosa strain ATCC 49129]
ATGAAACAGTCGCTCCAGCTCCGCCTCTCTCAGCATCTTGCGCTCACGCCGCAGTTGCAGCAGTCCATCCGGCTGCTGCA
GTTATCGACCATCGAACTGCAGCAGGAAGTTGAACAGGCACTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACCTCCGCGCGCATTGGCGCGGACGGCTCCGTCAACGCGCTGCAGAACAACAACGCCACGCCGCTGCCCACCGAA
TCGCCGCCGCCTTCGCCCAACGGGGCGGACAGTTCCGACACCCCCGACAACAGCTACGACGGCCACGACAGCGGCGCCGA
TTTCGACAGCGATTACGGCTCAGACCGCTCCGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCGGATGAAGACG
ATCGCGCCCCGCTGCAGTTGCGCGAAGCCGAACAAAGCCTGCGCGAATTCCTCATGGAACAGCTCGCGCCGCTCAAGCTG
TCGATGCGCGACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGACGATGAGGGCTATCTCTCCGCTACGCTCGACGA
AGTCCTCACCGACCTGCCCGCCGAACTCGAAGTCGAACTCGATGAGCTGCAGGCCGCGCTGCGCATGCTGCAGAGTTTCG
ATCCACCGGGCGTCGGTGCACGCTCGGCAGCCGAATGCCTGTCGCTGCAGTTGCATCGACTTGATTCACCGGCGAAGGCG
CTTGCGCTCATCATCGTCAACCAGCATCTCGAACTGCTTGCCGCACGCGACTACACGCGACTGAAGAAGGCGCTGTCCGT
CGATGAACCCGCGCTGAAGGCAGCGCATGAACTGATCCGTTCGTTGGCGCCGTTCCCCGGACACGCTTTCGGTCGGGCAG
AAGCCGACTTCGTGGTGCCCGACGTCGTCGTGCGCAAGACCAGTGCTGGCTGGATGGCACAACTCAATCCGGACGTCATG
CCGCGCCTGCGCATCAACGACATGTACGCGCAAATTCTGCGCAGCAGTCGTGGGGAAACCGGTGCCGCCAATCTGCAGCA
GAAACTGCAGGAAGCTCGTTGGCTGATCAAGAACATTCAGCAGCGCTTCGACACGATCCTGCGTGTTTCGCAAGCTATTG
TCGAACGTCAAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCAACGCCCATGGGGACGTTTGAGCTGAAGTA
CTTCTTCGGCAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTG
TAGGAGCCGAAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGTGAACAAGGATTCGTGGTGGCC
CGTCGGACGGTGGCCAAATACCGCGAAGCCCTCAAGATTCCTGCAGTGAATCTGCGCAAGTCTTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-500)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A191ZT41

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

92.231

100

0.922

Multiple sequence alignment